非小细胞肺癌组织中CRT、BAP31表达变化及其意义

Expression changes of CRT and BAP31 in non-small-cell lung cancer and its clinical significance

目的观察非小细胞肺癌(NSCLC)组织中钙网蛋白(CRT)和B细胞受体相关蛋白31(BAP31)的表达变化,并探讨其意义。方法手术切除的NSCLC组织60例份(肿瘤组)、癌旁(至少距肿瘤边缘5 cm以上)组织30例份(癌旁组),采用免疫组化法检测两组CRT、BAP31,并分析两指标间及与NSCLC临床病理参数的关系。结果肿瘤组、癌旁组CRT阳性率分别为88.33%、13.33%,BAP31阳性率分别为83.33%、6.67%,肿瘤组与癌旁组比较,P均<0.05。CRT阳性表达与NSCLC淋巴结转移、临床分期相关(P均<0.05)。肿瘤组CRT与BAP31表达无相关性(r=0.400,P>0.05)。结论 CRT和BAP31在NSCLC中均呈高表达,CRT的表达与临床分期和淋巴结转移相关,其可能参与NSCLC的发生发展。

Objective To observe the expression changes of calreticulin(CRT)and B cell receptor-associated protein31(BAP31)in the non-small-cell lung cancer(NSCLC)tissues and to explore their significance.Methods Sixty cases of NSCLC tissues from surgical resection(tumor group)and 30 cases of paracancerous tissues(at least 5 cm from the margin of the tumor,paracancerous group)were randomly selected.The expression of CRT and BAP31 in these two groups were determined by immunohistochemical method,the correlation between the expression of these two proteins and with the clinical pathological parameters of NSCLC were analyzed,respectively.Results The positive rates of CRT in the tumor group and paracancerous group were 88.33%and 13.33%,respectively;and the positive rates of BAP31 were 83.33%and6.67%,respectively(all P<0.05).The positive expression of CRT was related to lymph node metastasis and clinical stage of NSCLC(both P<0.05).In the tumor group,CRT was not related to BAP31 expression(r=0.400,P >0.05).Conclusion Both CRT and BAP31 are highly expressed in NSCLC,and the expression of CRT is related to clinical stage and lymph node metastasis,which may be involved in the occurrence and development of NSCLC.