唾液酸苷酶与脯氨酸氨基肽酶在细菌性阴道病中应用分析

Application of sialidase and proline aminopeptidase in bacterial vaginosis diagnosis

目的分析唾液酸苷酶与脯氨酸氨基肽酶在细菌性阴道病中的辅助诊断价值。方法收集2017年1月1日至11月13日因不孕不育来我院进行分泌物检查的29 279例标本,使用Nugent评分法鉴定细菌性阴道病,同时对每例标本进行唾液酸苷酶与脯氨酸氨基肽酶检测。结果 Nugent评分法鉴定出3938例BV阳性,阳性率为13.44%,在细菌性阴道病中唾液酸苷酶、脯氨酸氨基肽酶的检出率显著低于两者联合检测的检出率(P<0.05)。在细菌性阴道病中,唾液酸苷酶的敏感度、特异性、阳性预测值、阴性预测值分别为45.04%、99.85%、97.90%、92.12%,脯氨酸氨基肽酶的敏感度、特异性、阳性预测值、阴性预测值分别为30.65%、99.91%、98.20%、90.26%,唾液酸苷酶+脯氨酸氨基肽酶联合检测的敏感度、特异性、阳性预测值、阴性预测值分别为54.76%、99.81%、97.91%、93.42%。结论唾液酸苷酶与脯氨酸氨基肽酶对细菌性阴道病均有辅助诊断意义,且有互补作用,两者联合检测对细菌性阴道病更有诊断价值。

Objective To analyze the assistant diagnostic value of sialidase and proline aminopeptidase in bacterial vaginosis.Methods 29 279 specimens were collected in our hospital from January 1 to November 13,2017 for secretion examination due to infertility.Bacterial vaginosis(BV)was identified by Nugent scoring method.Meanwhile,sialidase and proline aminopeptidase were detected in each specimens.Results Nugent scoring method have identified 3938 BV positive cases,the positive rate was 13.44%.In these BV cases,the detection rate of sialidase and proline aminopeptidase was significantly lower than that of the two(P<0.05).The sensitivity,specificity,positive predictive value and negative predictive value of sialidase for BV were 45.04%,99.85%,97.90%and 92.12%,respectively.The sensitivity,specificity,positive predictive value and negative predictive value of proline aminopeptidase were 30.65%,99.91%,98.20%and 90.26%,respectively.The sensitivity,specificity and negative predictive value of combined detection of sialidase and proline aminopeptidase were 30.65%,99.91%,98.20%and 90.26%,respectively.Positive predictive value and negative predictive value were 54.76%,99.81%,97.91%and 93.42%respectively.Conclusion Both sialidase and proline aminopeptidase are helpful in the diagnosis of BV,and they complement each other.The combined detection of sialidase and proline aminopeptidase is more valuable in the diagnosis of BV.