MG-132对TRAIL诱导骨肉瘤细胞U-2 OS凋亡的影响

Influence of MG-132 on TRAIL inducing apoptosis of osteosarcoma cell U-2 OS

目的探讨MG-132对TRAIL诱导骨肉瘤细胞U-2 OS凋亡的影响。方法骨肉瘤细胞U-2 OS置于含10%小牛血清的1640培养液中,在37℃、5%CO_2饱和湿度的培养箱中培养,分为单用TRAIL组、单用MG-132组、联合组。在应用上述三种给药方案24 h后,分别对诱导MG-132细胞凋亡状况进行检测;计算细胞抑制率;光镜下观察细胞变化。结果单用TRAIL组诱导凋亡效果并不理想,随着TRAIL浓度的升高,骨肿瘤U-2 OS凋亡率不断升高,单用MG-132组细胞随着给药的浓度增加,凋亡率也随之增高,差异有统计学意义(P<0.05);联合组、单用TRAIL组和单用MG-132组分别与空白对照组比较,骨肉瘤细胞凋亡率均明显升高,差异有统计学意义(P<0.05),且单用MG-132组高于单用TRAIL组,差异有统计学意义(P<0.05),联合组骨肉瘤细胞凋亡率明显高于单用TRAIL组和单用MG-132组,差异有统计学意义(P<0.05)。随着时间的推移,细胞抑制率上升,而联合组的抑制率明显高于TRAIL组和MG-132组,在72 h处达到最大值[(82.32±2.54)%],差异有统计学意义(P<0.05)。结论单用TRAIL可诱导骨肉瘤细胞U-2 OS凋亡,但效果不太理想。联合MG-132使用后,可大大提高TRAIL诱导U-2OS凋亡的效果。

Objective To discusses the influence of MG-132 on TRAIL inducing apoptosis of osteosarcoma cell U-2OS.Methods Osteosarcoma cells U-2OS were cultured in 1640 culture medium containing 10%calf serum in a incubator at 37℃and 5%CO2 saturation humidity.They were divided into single-use TRAIL group,single-use MG-132 group and combined group.After 24 hours of application of the above-mentioned three dosage regimens,and the apoptotic status of MG-132 cells was detected,the cell inhibition rate was calculated,and the cell changes were observed under light microscope.Results The effect of apoptosis induction in the single-use TRAIL group was not ideal.With the increase of TRAIL concentration,the apoptosis rate of U-2 OS of bone tumor was continuously increased.The apoptosis rate increased with the increase of drug concentration in single-use MG-132 group,and the difference was statistically significant(P<0.05).The apoptotic rate of osteosarcoma cells in combined group,single-use TRAIL group and single-use MG-132 group was significantly higher than that in blank control group,and the difference were statistically significant(P<0.05),and single-use MG-132 group was higher than single-use TRAIL group,and the difference was statistically significant(P<0.05),and the apoptotic rate of osteosarcoma cells in combined group was significantly higher than that in single-use TRAIL group and single-use MG-132 group,and the difference was statistically significant(P<0.05).With the passage of time,the cell inhibition rate increased,and the inhibition rate of combined group was significantly higher than that of single-use TRAIL group and single-use MG-132 group,the maximum value reached at 72 hours([82.32±2.54]%),and the difference was statistically significant(P<0.05).Conclusion Single-use TRAIL can induce apoptosis of osteosarcoma cell U-2 OS,but the effect was not ideal and after combining use of MG-132,it can significantly improve the effect of TRAIL induced apoptosis of U-2 OS.