阻塞性睡眠呼吸暂停伴认知障碍患者中血清miR-132表达及意义

Expression and Significance of Serum Mir-132 in Patients with Obstructive Sleep Apnea with Cognitive Impairment

【目的】探讨miR-132在阻塞性睡眠呼吸暂停(OSA)伴认知障碍患者血清中的表达及其意义。【方法】根据蒙特利尔认知评估(MoCA)评分,将66名年龄在30～60岁之间的阻塞性睡眠呼吸暂停患者分为两组:认知障碍OSA组(OSAI组,n=36)、无认知障碍OSA组(OSAN组,n=30),30名无OSA的成年人作为对照组(HC组,n=30)。对其3组分别进行睡眠测试(OCST)和MoCA评估,并通过PCR检测血清中miR-132的相对表达量。【结果】HC组、OSAI组及OSAN组在年龄、教育程度、性别和高血压方面差异无统计学意义(P>0.05),与OSAN和HC组相比,OSAI组患者血清中miR-132的相对表达水平明显上调(P<0.001),并且与MoCA评分呈正相关(r=-0.726,P<0.001);ROC分析结果显示曲线下面积(AUC)具有统计学意义,具有认知功能障碍(AUC=0.935,95%CI:0.890-0.981,P<0.001)和OSA(AUC=0.787,95%CI:0.695-0.879,P<0.001)。【结论】血清miR-132表达水平升高与OSA的诊断及其认知功能障碍密切相关。血清miR-132的检测有可能成为OSA患者认知功能障碍及诊断的一个潜在指标。

【Objective】To explore the relationship between the miR-132 expression in serum and cognitive deficits of OSA.【Methods】66 Chinese adults age 30 to 60 years old were enrolled and categorized into two groups based on Montreal Cognitive Assessment(MoCA)scores:OSA patients with cognitive impairment(OSAI,n=36),OSA patients without cognitive impairment(OSAN,n=30),and thirty adults without OSA as healthy control group(HC,n=30).Outof-center cardiopulmonary sleep testing(OCST)and MoCA assessment were performed and the relative expression of miR-132 in serum was detected by PCR.【Results】No significant difference was observed in age,education,gender and hypertension(P >0.05).The relative expression level of miR-132 was significantly up-regulated in OSAI patient′s serum compared to the OSAN and HC patients(P<0.001),and had a positive correlation with MoCA score(r=-0.726,P<0.001).ROC analyses showed that the areas under the curve(AUC)were statistically significant from the line of identity in OSA with cognitive impairment(AUC=0.935,95%CI:0.890-0.981,P<0.001)and in OSA(AUC=0.787,95%CI:0.695-0.879,P<0.001).【Conclusions】Elevated serum miR-132 expression levels are closely related to the diagnosis of OSA and its cognitive dysfunction.Detection of serum miR-132 may be a potential indicator of cognitive dysfunction and diagnosis in OSA patients.