壳聚糖和普朗尼克介导猪白细胞介素-23基因体内表达对PRRSV疫苗的免疫增强效应

Enhancement of immune responses to PRRSV vaccine by in vivo expression of porcine IL-23 gene mediated by Chitosan and Pluronic

猪繁殖和呼吸综合征(PRRS)是一种严重影响养猪业的高度传染性疾病,目前预防这种疾病的疫苗免疫还存在一定缺陷。本研究旨在探索一种有效的佐剂以提高其免疫效力。将猪IL-23基因重组质粒用壳聚糖和普朗尼克材料包裹制成纳米颗粒,命名为VRIL23-CNP-Pluronic。将28日龄小鼠分为两组,分别肌肉注射VRIL23-CNP-Pluronic(实验组)和VR1020-Pluronic(对照组),两组均同时接种PRRSV疫苗,在接种后第0、7、14、28和35天采集血样并分析免疫变化。实验组中PRRSV特异性抗体、IgG1和IgG2a水平、CD4+和CD8+T淋巴细胞数量均极显著高于对照组(p<0.01);经qRT-PCR分析,与对照组相比,实验组小鼠的IL-23、TLR1、TLR6、STAT1、IL-10、TNF-α、IL-15和CD62L基因表达水平均极显著上调(p<0.01)。结果表明,VRIL23-CNP-Pluronic能促进小鼠对PRRSV疫苗的免疫应答,并为其作为新型PRRSV疫苗佐剂的开发提供理论基础。

Porcine reproductive and respiratory syndrome(PRRS)is a severe,economically important infectious disease of pigs.Current vaccines to control the disease,however,are inadequate due to low immunity;and the present study was conducted to explore an effective adjuvant to enhance the immunological efficacy.A recombinant plasmid containing the porcine IL-23 gene was encapsulated in chitosan nanoparticles and Pluronic-L64 designated as VRIL23-CNP-Pluronic,which was tested following intramuscular injection of 28 day-old mice in comparison with VR1020-Pluronic as the control.Over a 35 day observation period,PRRSV-specific antibody,IgG1 and IgG2a titers,CD4^+and CD8^+T cells increased significantly in the treated mice(P<0.01).As analyzed by qRT-PCR,expression levels of IL-23,TLR1,TLR6,STAT1,IL-10,TNF-α,IL-15 and CD62L genes were also significantly up-regulated in comparison with those of control mice(P<0.01).These results show that co-injection of VRIL23-CNP-Pluronic with PRRS vaccine enhances both innate and adaptive immunity of mice,and provide support for its development as a novel vaccine adjuvant for control of swine PRRS.