利用生物信息学探索介导鲍曼不动杆菌血流感染的相关蛋白

Exploring on related proteins mediating Acinetobacter baumannii bloodstream infection based on bioinformatics

目的探索介导鲍曼不动杆菌(AB)血流感染的相关蛋白,为AB血流感染提供新的研究方向。方法在AB基因数据库中检索多位点序列分型(MLST)相同,分离部位不同的AB菌株,采用生物信息学的方法分析仅存在于血流感染AB中的差异蛋白,并进行功能注释分析。结果在设定的检索条件下,共检索10株AB,按MLST分为3组,其中ST92型4株(分离自血、痰各2株)、ST137型4株(分离自血、痰各2株)、ST365型2株(分离自血、痰各1株)。基因、蛋白数据库分析结果显示,部分蛋白仅存在于血流感染AB中,不存在于分离自痰的AB中,其中ST92、ST137、ST365型血流感染AB中分别存在20、4、22个特有蛋白。特有蛋白参与的生物学过程主要集中在:细菌毒力与侵袭力、细菌代谢、DNA合成、DNA损伤及修复、转录、翻译、基因转座、以及噬菌体相关蛋白等通路上。结论由于个体差异性,同一MLST的AB上述蛋白表达的差异,可能是导致其发生血流感染的原因。

Objective To explore the related protein mediating Acinetobacter baumannii(AB)bloodstream infection(BSI),provide a new research direction for AB bloodstream infection(BSI).Methods AB strains with the identical multilocus sequence typing(MLST)but isolated from different sites were retrieved from AB gene database,related differential protein which only existed in AB of BSI were analyzed by bioinformatics,and functional annotation analysis was conducted.Results Under the established retrieval conditions,a total of 10 strains of AB were retrieved and divided into three groups according to MLST,including 4 strains of ST92(2 strains from blood,and 2 from sputum),4 strains of ST137(2 from blood,and 2 from sputum),and 2 strains of ST365(1 from blood,and 1 from sputum).Gene and protein database analysis results showed that partial protein only existed in AB of BSI,didn’t exist in AB isolated from sputum,there were 20,4,and 22 specific protein in AB causing ST92,ST137,and ST365 BSI,respectively.The biological process in which specific protein involved was mainly concentrated on bacterial virulence and invasiveness,bacterial metabolism,DNA synthesis,DNA damage and repair,transcription,translation,gene translocation,and phage-related protein.Conclusion Because of individual difference,AB of the same MLST expressing the above differential protein may be the cause of BSI.