异源表达TaEDR1抑制拟南芥edr1白粉病抗性

Heterologous Expression of TaEDR1 Inhibits Edr1 Powdery Mildew Resistance in Arabidopsis

EDR1是一个植物抗性相关基因,编码一个Raf样MAPKKK蛋白激酶,通过调节MAPK激酶级联通路调控植物免疫反应。本研究获得了小麦TaEDR1基因的全长cDNA序列,构建进入过表达植物双元载体pBI1.4t,利用农杆菌介导法将CaMV35S过表达启动子驱动的TaEDR1基因(35S-TaEDR1-pBI1.4t)导入拟南芥edr1突变体中,并利用白粉菌对转基因和野生型拟南芥进行接种处理。结果表明:TaEDR1的异源表达能够抑制分生孢子梗生长少、过氧化氢大量积累和病程相关基因At PR1大幅上调表达等edr1突变体抗白粉病表型。为今后EDR1基因抗病的功能研究及在其他经济作物中的应用奠定基础。

EDR1 is a plant resistance-related gene encoding a Raf-like MAPKKK protein kinase,which regulates MAPK immune response through regulating the level of MAPK kinase cascade.In this study,full-lengthen cDNA sequence of wheat TaEDR1 gene was cloned,and the plant over-expression vector 35S-TaEDR1-pBI1.4t was introduced into edr1 mutant of Arabidopsis,and the transgenic Arabidopsis and wild type ones were inoculated by powdery mildew.The results showed that heterologous expression of TaEDR1 could effectively inhibit the phenotype of powdery mildew resistance,such as less growth of the conidia,great accumulation of hydrogen peroxide and up-regulation expression of AtPR1 in edr1 mutant.It will lay the foundations for future research on the function of EDR1 gene and its application in other economic crops.