山羊副流感病毒3型感染MDBK细胞的转录组分析

Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain

本研究旨在探究山羊副流感病毒3型(CPIV3)感染MDBK细胞后的转录组基因变化情况,丰富CPIV3转录组信息。取1MOI CPIV3JSHA2014-1病毒液感染MDBK细胞,设非感染正常细胞为对照,于24h后收获细胞,提取总RNA,利用Illumina HiSeqTM2500对感染组与对照组进行高通量测序,并用测序评估、基因注释等生物信息学方法进行分析。结果显示,差异表达基因共261个,其中表达上调140个,表达下调121个,经RT-qPCR方法验证8个差异表达的干扰素信号通路相关基因,结果与高通量测序一致。进一步GO分类结果显示,差异表达基因主要涉及细胞生物学进程、构成细胞的组分以及实现的分子功能三个方面,KEGG分析显示这些基因参与代谢、生物系统、细胞进程、基因信息进程和环境信息进程。本研究为深入探究CPIV3的致病机制奠定了基础。

To explore the cellular transcriptional gene differentially expressed in MDBK cells infected with caprine parainfluenza virus type 3(CPIV3),we analyzed the mRNA expression profiles in MDBK cells infected with 1 MOI CPIV3 JSHA2014-1 strain at 24 hpi using high-throughput sequencing.The results indicated that a total of 261 genes were obviously differentially expressed in the infected MDBK cells including 140 up-regulated genes and 121 down-regulated genes.The eight of these genes were verified by RT-qPCR assay,and the results were consistent with those of high-throughput sequencing.GO analysis classification showed that differentially expressed genes were mainly involved in biological processes,cellular components and molecular functions.KEGG analysis shows that the signaling pathways involved in these genes included metabolism-related signaling pathways,biological systems,cellular processes,gene information processes and environmental information processes.This study laid the foundation for further exploring the pathogenesis of CPIV3.