窖泥中高产愈创木酚类功能菌的筛选与鉴定

Screening and identification of functional boateria with high yield guaiacol in the mud

试验以黄鹤楼酒浓香型窖泥为材料,采用传统微生物分离方法即稀释涂布平板法对样品进行涂布,然后平板划线纯化、镜检,单菌发酵利用GC-MS筛选出高产愈创木酚类物质的功能菌株,然后利用分子生物学技术对菌种进行鉴定,最后进行菌种的耐受性分析。试验结果为:筛选出2株高产愈创木酚类物质的功能菌B-1、B-3,经鉴定分别为Bacillus amyloliquefaciens、Delftia sp. X-a12,总产量分别为3 632. 33和14 527. 31μg/L。耐受性分析结果为菌株B-1、B-3均耐4%vol酒精度、pH 4. 5、15%糖度。

The experiment was carried out using dilution coating method to coat the mud of the Yellow Crane Tower Luzhou-flavor liquor.Plate scribing purification,microscopic examination,and single-bacteria fermentation were conducted,followed by using gas chromatography-mass spectrometer(GC-MS)to screen functional bacteria with high yield of guaiacol.The results of molecular biology techniques showed that two functional strains,B-1 and B-3,with high-yield of guaiacol were Bacillus amyloliquefaciens and Delfitia sp.X-a12,respectively.Their total yields were 3 632.33μg/L and 14 527.31μg/L,respectively.The tolerance analysis results showed that both strains were resistant to 4%vol.alcohol,pH=4.5,and 15%sugar.