黄原胶支持CIK细胞高密度扩增

CIK cells expansion in high density culture containing xanthan gum

基于细胞因子诱导杀伤细胞(CIK)的细胞疗法是癌症治疗的关注热点之一,获得足够数量具有功能的效应细胞是临床应用的重要环节,而实现效应细胞高密度培养,减少培养体积,是降低治疗成本的有效途径。黄原胶作为一种信号分子,具有调控细胞增殖的活性。为此,以外周血单个核细胞为研究对象,以总细胞扩增倍数、效应细胞比例和扩增倍数,以及扩增后CIK细胞对K562细胞的杀伤活性为评价指标,考察了黄原胶对CIK细胞体外高密度扩增的影响。结果显示:当接种密度为4×106 cells×mL^(-1)时,在含有100μg×mL^(-1)黄原胶的无血清培养基中,总细胞扩增倍数可达到150.0±29.1,显著高于不含黄原胶对照组的41.1±5.1(p<0.05);CD3+CD56+细胞的比例和扩增倍数分别为(16.7±7.6)%和321.4±48.6,显著高于对照组的(13.2±6.8)%和71.4±31.3(p<0.05);而且添加黄原胶时扩增后CIK细胞对K562细胞杀伤活性的均值可从28.7%增加到34.3%。该研究结果可为CIK细胞体外扩增过程的优化提供技术支持。

Cell therapy based on cytokine-induced killer(CIK)cells is one of the hot areas in cancer treatment.Sufficient functional effector cells are needed for clinical application and high density culture is an effective way to reduce treatment costs.Xanthan gum(XG)as a signaling macromolecule can regulate cell proliferation.Effects of XG on in vitro high density expansion of CIK cells derived from peripheral blood was investigated by analyzing expansion fold of total cells,proportion and expansion fold of effector cells and cytotoxicity of CIK cells against K562 cells.The results indicate that when the culture density is 4×10 6 cells·mL^-1,total cell expansion is 150.0±29.1 folds in serum-free medium with 100μg·mL^-1 XG,which is significantly higher than that of the control group(41.1±5.1 folds,p<0.05).The proportion and fold expansion of CD3^+CD56^+cells are(16.7±7.6)%and 321.4±48.6 folds,respectively,which are also significantly higher than those of the control group((13.2±6.8)%and 71.4±31.3 folds,p<0.05).Furthermore,mean cytotoxicity of expanded CIK cells against K562 cells increases from 28.7%to 34.3%with the addition of 100μg·mL^-1 XG.These results provide technical supports for the optimization of CIK cells expansion in vitro