植物组织培养过程中的DNA甲基化研究进展

Research Progress on DNA Methylation During Plant Tissue Culture

组织培养是植物无性繁殖和遗传转化的重要技术基础。组织培养包括外植体脱分化、愈伤组织形成、再分化等过程,最后形成新的植株。在植物组织培养过程中,DNA甲基化模式不断发生变化,表明植物细胞发生了大量的表观遗传重编程事件。一般来说,DNA甲基化可以促进或加快组织培养过程,对于许多很难进行组织培养和再生的植物来说非常重要。本文主要总结了植物组织培养过程中DNA甲基化模式的变化规律和分子机制,以及新的研究手段和思路,旨在为开展植物组织培养过程中的DNA甲基化研究提供帮助。

Tissue culture is the important technical basis for plant asexual propagation and genetic transformation. Tissue culture includes explants dedifferentiation, callus formation, callus redifferentiation and plantlets formation. DNA methylation pattern is constantly changing during tissue culture, which means many epigenetic reprogramming events occur in cells. Generally speaking, DNA methylation can promote or accelerate the tissue culture process, it’s very important for many plants that are difficult to regenerate. The paper mainly summarizes the pattern change and molecular mechanism of DNA methylation during plant tissue culture, as well as new methods and ideas. We hope the paper could help the study of DNA methylation in plant tissue culture.