塞来昔布对人骨肉瘤MG-63细胞增殖、凋亡及PI3K/Akt信号通路的影响

Effect of Celecoxib on the Proliferation and Apoptosis of MG-63 Cells Through PI3K/Akt Pathway

目的观察COX-2选择性抑制剂塞来昔布对人骨肉瘤细胞株MG-63的增殖、凋亡及PI3K/Akt信号通路的影响。方法对数生长期人骨肉瘤细胞株MG-63细胞用不同浓度的塞来昔布溶液(0、25、50和100μmol/L)处理后,MTT比色法检测24 h、48 h及72 h各组细胞增殖活性的影响;流式细胞仪测定各组细胞凋亡率; Western blot检测COX-2、CAS-3和P-Akt蛋白表达变化。结果塞来昔布对MG-63细胞具有抑制增殖的作用,且该作用与药物浓度及作用时间呈正相关;流式细胞术检测结果显示细胞凋亡率与塞来昔布浓度呈正相关;随着塞来昔布浓度的增加,COX-2和P-Akt蛋白的表达水平逐渐降低,而CAS-3表达水平逐渐升高。结论塞来昔布抑制MG-63细胞增殖,促使细胞凋亡,其机制与下调PI3K/Akt信号通路有关。

Objective To explore the effect of Celecoxib on the proliferation and apoptosis of MG-63 cells,and elucidated the correlation between the effect and PI3K/Akt pathway.Methods Cultured MG-63 cells were treated with different concentration of Celecoxib(0,25,50 and 100μmol/L),MTT assay was used to test the cell proliferation,and the rates of apoptosis of cells were assessed by the flow cytometry.Western blot were emplored to examine the expression of COX-2,CAS-3 and P-Akt in cultured MG-63 cells.Results Celecoxib could inhibit the proliferation of MG-63 cells significantly in a dose and time dependent manner,and compared to control,the apoptosis rate significantly increased with increases in concentrations of celecoxib;Furthermore,The level of COX-2,CAS-3 and P-Akt protein expression were significantly down-regulated with increases in concentrations of celecoxib(P<0.05).Conclusion The inhibition of proliferation and apoptosis in MG-63 cells could be induced significantly,and its mechanism might be the down regulation of the expression of PI3K/Akt pathway.