厦门地区低致病性禽流感病毒流行病学调查与分析

Epidemiological survey and analysis of low pathogenic avian influenza viruses in Xiamen, China

目的了解厦门地区家禽低致病性禽流感病毒的流行情况。方法本研究选取禽流感病毒检出率最高的冬春季节,从厦门市几家主要活禽交易市场采集家禽棉拭子及环境样品,用荧光定量PCR方法进行低致病性禽流感病毒的检测和分型鉴定。结果从534份样品中,检测出低致病性禽流感病毒阳性216份,检出率为40.4%。本地低致病禽流感病毒的HA亚型至少6种,检出率由高到低依次为H9、H6、H11、H12、H1、H3;NA亚型至少5种,检出率由高到低依次为N2、N6、N3、N8、N9。结论活禽交易市场家禽中存在不同亚型混合感染情况,尤其以水禽最明显,可同时检出多种亚型。提示今后厦门的疫病防控须加大对水禽的监测及交易市场的管理和消毒。

This study engaged in epidemiological surveillance of low pathogenic avian influenza A viruses in poultry of Xiamen.From winter to spring,cloacal swab samples were collected from poultry,which were from main live-poultry markets of different districts in Xiamen.Real-time PCR method were using for detection and subtype identification.The 216 strains of AIVs were detected from 534 cloacal swab samples,with a detection rate of 40.4%.These strains of AIV belonged to 6 HA subtypes and 5 NA subtypes,the detection rate of high to low were H9,H6,H11,H12,H1,H3 and N2,N6,N3,N8,N9.Mixed infections were much higher in waterfowl origin,different subtypes were detected in live-poultry markets,indicating that monitoring of avian influenza virus in domestic waterfowls should be reinforced an standardized administration and thoroughly disinfection were needed in future.