麦门冬汤对大鼠骨髓间充质干细胞向肺泡上皮细胞分化的影响

Effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC) to differentiate into alveolar epithelial cells of rat

[目的]观察麦门冬汤含药血清对促进大鼠骨髓间充质干细胞(BMSC)向肺泡上皮细胞分化的影响。[方法]分别予麦门冬汤大鼠血清、肺纤维化模型大鼠血清、肺纤维化模型大鼠+麦门冬汤血清对绿色荧光蛋白标记的骨髓间充质干细胞(GFP-BMSC)进行干预14 d,通过免疫组化、实时荧光定量聚核酶链反应(qPCR)方法,检测各组GFPBMSC细胞肺表面活性蛋白(SP-C)、水通道蛋白-5(AQP-5)蛋白及mRNA的表达。[结果]模型组、模型+麦门冬汤组SP-C、AQP5蛋白表达明显高于DMEM对照组、麦门冬汤组(P<0.01),其中模型+麦门冬汤组SP-C、AQP5表达明显高于模型组(P<0.01)。[结论]麦门冬汤在肺纤维化环境中可促进GFP-BMSC向肺泡上皮细胞分化,该作用可能是其改善肺纤维化的重要机制。

[Objective]To observe the effect of Maimendong Decoction on bone marrow mesenchymal stem cells(BMSC)to differentiate into alveolar epithelial cells of rats.[Methods]Maimendong Decoction rat serum,rat model of pulmonary fibrosis in rats serum and rat model of pulmonary fibrosis+Maimendong Decoction serum were given on GFP-BMSC.After 14 days,to detect the expression of SP-C,AQP-5 protein and mRNA in GFP-BMSC cells by immunohistochemistry and qPCR method.[Results]The expression of SP-C and AQP-5 proteins in the model group,model+Maimendong Decoction group was significantly higher than that of DMEM control group,Maimendong Decoction group(P<0.01);while the model+Maimendong Decoction group SP-C,AQP-5 proteins expression was significantly higher than the model group(P<0.01).[Conclusion]Maimendong Decoction can promote GFP-BMSC cells into alveolar epithelial cells in pulmonary fibrosis,which may be one of the important mechanisms to improve pulmonary fibrosis.