基于UHPLC-Q-TOF-MS/MS的白芍炒制前后化学成分研究

Qualitative Analysis of Major Constituents of Raw and Processed Paeonia Lactiflora by UHPLC-Q-TOF-MS/MS

目的:采用超高效液相色谱串联四极杆飞行时间质谱法,探讨白芍炒制前后化学成分的变化。方法:采用AgilentC18色谱柱,0. 1%甲酸水溶液-乙腈梯度洗脱,体积流量0. 3 m L/min,串联四级杆飞行时间质谱分别测定不同产地各10批生、炒白芍中的化学成分。结果:分别在生、炒白芍中鉴定出40种化学成分。结论:通过UHPLC-Q-TOF-MS/MS技术,为分析生、炒白芍中的化学成分提供一种快速、高效的分析方法,为综合评价生白芍和炒白芍的质量提供参考。

Objective:By using ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry,to investigate the changes of chemical composition in Paeonia lactiflora before and after progressed.Methods:Agilent C 18 column,0.1%formic acid aqueous solution-acetonitrile gradient elution,volume flow rate 0.3 mL/min,tandem four-stage rod time-of-flight mass spectrometry were used to determine the chemical composition of 10 batches of raw and fried white peony in different producing areas.Results:A total of 40 chemical components were identified in raw Paeonia lactiflora(RPL)and processed Paeonia lactiflora(PPL).Conclusion:UHPLC-Q-TOF-MS/MS technology provides a fast and efficient analytical method for the analysis of chemical constituents in RPL and PPL,which provides a reference for comprehensive evaluation of the quality of RPL and PPL.