MicroRNA-197调控上皮-间充质转化对乳腺癌侵袭和迁移的影响

Effects of microRNA-197 regulating epithelial mesenchymal transition on invasion and migration in breast cancer

目的:探讨微小RNA-197(miR-197)抑制乳腺癌细胞迁移和侵袭的能力,以及阻断上皮-间充质转化(epithelial-mesenchymal transition,EMT)过程的机制。方法:构建miR-197过表达载体(miR-197mimics),分别转染MDA-MB-231和MCF-7细胞,并设对照组;Real-timePCR分别检测以上各组细胞miR-197的表达水平变化;利用Transwell实验和划痕实验对乳腺癌细胞侵袭和迁移能力进行检测;Western印迹法检测过表达miR-197后对EMT相关标志物E-cadherin,snail和vimentin表达的影响。结果:MiR-197可以抑制乳腺癌细胞MDA-MB-231和MCF-7的迁移和侵袭能力;转染miR-197mimics后,E-cadherin表达降低,snail和vimentin表达增加。结论:MiR-197有可能作为乳腺癌临床治疗的新靶点。

Objective: To investigate the ability of microRNA-197 (miR-197) to inhibit the migration and invasion of breast cancer cells and the mechanism of blocking the process of epithelial-mesenchymal transition (EMT). Methods: The miR-197 mimics vector was constructed, and transfected into MDA-MB-231 and MCF-7 cell, the control group were transfected with empty vectors. The expression of miR-197 was detected by real-time PCR. The transwell system and the wound healing were used to assess the invasion and migration ability of breast cancer cell line MDA-MB-231 and MCF-7. Western blot was used to assay the expression of E-cadherin, Snail and Vimentin, which were the markers of EMT. Results: MiR-197 inhibited the migration and invasion of breast cancer cells MDA-MB-231 and MCF-7. Transfection of miR-197 mimics reduced the expression of E-cadherin and increased the protein expression of snail and vimentin. Conclusion: MiR-197 may be a new target for clinical treatment of breast cancer.