摘要
通过水提醇沉法得到西洋参果中总多糖(WQBP),再通过离子交换层析分离纯化,得到中性糖WQBP-N和酸性糖WQBP-A。利用高效液相色谱法对单糖组成进行分析,并检测各级分的1,1-二苯基-2-二硝基苯肼(DPPH)自由基清除活性。结果表明,WQBP主要由鼠李糖(Rhamnose,Rha)、半乳糖醛酸(Galacturonic acid,GalA)、半乳糖(Galactose,Gal)、阿拉伯糖(Arabinose,Ara)和葡萄糖(Glucose,Glc)组成;WQBP-N主要由Gal、Ara、Glc和甘露糖组成,比例为63.9∶19.3∶15.2∶1.5;WQBP-A主要由Rha、GalA、Gal、Ara和Glc构成,比例为13.1∶13.2∶46.4∶19.0∶5.8。WQBP对DPPH自由基清除活性优于其子级分,并且清除率随着浓度的升高而增加。研究结果能够为西洋参果多糖的开发利用提供一定的数据基础。
Extracted and purified polysaccharide fractions from Panax quinquefolius berry,and DPPH radicals scavenging activity was carried on.Polysaccharide fractions(WQBP)from P.quinquefolius berry was dealed with water-extraction,ethanol-precipetation and ion-exchange chromatography,then one neutral(WQBP-N)and one acidic fraction(WQBP-A)were obtained.The monosaccharide composition,molecular weight distribution and DPPH free radicals scavenging activity were analyzed.WQBP is mainly composed of rhamnose,galacturonic acid,galactose,arabinose and glucose.WQBP-N is composed of galactose,arabinose,glucose and mannose with a ratio of 63.9∶19.3∶15.2∶1.5;WQBP-A is mainly composed by rhamnose,galacturonic acid,galactose,arabinose and glucose with a ratio of 13.1∶13.2∶46.4∶19.0∶5.8.WQBP has more significant DPPH free radicals scavenging activity than neutral and acidic fraction,which has a concentration dependant tendency.WQBP has potential antioxidant activity in vitro,and the results above could provide theory basis for the further application and developments of the P.quinquefolius berry polysaccharide.
作者
李珊珊
祁玉丽
曲迪
张磊
孙印石
LI Shan-shan;QI Yu-li;QU Di;ZHANG Lei;SUN Yin-shi(Institute of Special Wild Economic Animals and Plants,Chinese Academy of Agricultural Sciences,Changchun 130112,China;College of Chinese Medicinal Material,Jilin Agricultural University,Changchun 130118,China)
出处
《特产研究》
2019年第1期1-4,共4页
Special Wild Economic Animal and Plant Research
基金
吉林省科技发展计划医药产业发展引导资金项目(20180311037YY)
吉林省科技创新人才计划青年科研基金项目(20160520048JH)
