乌苏里瓦韦对小鼠镇咳、祛痰作用研究

Antitussive and Expectorant Effects of Lepisorus Ussuriensis in Mice

研究乌苏里瓦韦95%乙醇提取物对小鼠的镇咳、祛痰作用,并探讨是否通过抗炎机制而发挥药理作用。将ICR小鼠随机分为空白对照组、阳性药组、乌苏里瓦韦高剂量组(1.6 g/kg)、乌苏里瓦韦中剂量组(0.8 g/kg)、乌苏里瓦韦低剂量组(0.4 g/kg),分别采用氨水引咳法、气管酚红排泌法等经典模型研究乌苏里瓦韦的镇咳和祛痰作用,继而通过气管注射脂多糖造成急性炎症模型以探讨其抗炎机制。乌苏里瓦韦高、中剂量组均可显著延长小鼠的咳嗽潜伏期,减少咳嗽次数,增加气管酚红分泌量(P <0.01,P <0.05),并可明显降低小鼠IL-6、IL-1和TNF-的含量(P <0.01,P <0.05)。乌苏里瓦韦具有较好的镇咳、祛痰作用,其机制可能是通过降低IL-6和IL-1等血清炎性因子以及TNF-的含量发挥作用。该研究结果可为进一步开发利用乌苏里瓦韦提供理论数据支持。

To investigate the antitussive and expectorant effects of Lepisorus ussuriensis in mice,and to discuss the anti-inflammatory mechanism.Mice were randomly divided into control group,positive drug group,high,medium and low dose L.ussuriensis groups(1.6 g/kg,0.8 g/kg,0.4 g/kg).The methods of ammonia cited cough,tracheal phenol red excretion and tracheal instillation LPS were respectively used in the study,and some index such as the cough incubation period and cough frequency,tracheal phenol red secretion,the levels of IL-6,IL-1βand TNF-αwere than determined.The incubation period of cough has been prolonged,the frequency of cough has been reduced and the secretion of phenol red has been increased significantly in the high and middle dosage groups of L.ussuriensis(P<0.05,P<0.01).The inflammatory cytokines such as IL-6,IL-1βand and TNF-αin serum were also decreased significantly(P<0.05,P<0.01)in these two groups.L.ussuriensis has the good antitussive and expectorant effects,and the anti-inflammatory effect may be one of the mechanisms.