胃肠道动力障碍的相关因子在家兔重症急性胰腺炎模型的变化

Changes of Related Factors of Gastrointestinal Motility Disorders in Rabbit Models of Severe Acute Pancreatitis

目的:研究重症急性胰腺炎(SAP)家兔引起胃肠道动力障碍的相关因子。方法:选择30只SPF级新西兰白兔随机分为对照组和实验组,每组各15只。实验组白兔通过注射胰管内牛磺胆酸钠,建立重症急性胰腺炎模型,对照组白兔操作同实验组,但注射等容积蒸馏水。术后24h,观察白兔腹腔内大体情况,计算胃肠传递指数(TI),并检测血清胃泌素(GAS)、胃动素(MTL)、P物质(SP)、血管活性肠肽(VIP)、CCK(胆囊收缩素)、肿瘤杀伤因子-α(TNF-α)、白介素-1(IL-1)及白介素-6(IL-6)浓度,取胰腺组织制作病理切片,显微镜下观察,并进行病理学评分(PSP)。结果:实验组白兔腹腔内可见大量浑浊腹水,多处皂化斑,胰腺大范围出血坏死,胃肠道明显充血水肿扩张伴有部分坏死,镜下可见胰腺组织叶间隙增宽,细胞水肿,部分腺泡细胞坏死,并有大量炎性细胞浸润。实验组TI明显低于对照组,PSP明显高于对照组,差异有统计学意义(P<0.05);实验组白兔GAS、SP和VIP浓度高于对照组,MTL、CCK浓度低于对照组,差异有统计学意义(P <0.05);实验组白兔TNF-α、IL-1及IL-6浓度均高于对照组,差异有统计学意义(P <0.05)。结论:SAP白兔存在胃肠动力障碍,与胃肠激素GAS、MTL、SP、VIP、CCK的异常表达影响胃肠道运动功能,以及炎性因子的TNF-α、IL-1及IL-6大量表达加剧胃肠道损伤有关。

Objective:To study the related factors of gastrointestinal motility disorder in rabbits with severe acute pancreatitis.Methods:30 New Zealand SPFrabbits were randomly divided into control group and experimental group,15 rabbits in each group.The rabbits in the experimental group were established by injection of sodium taurocholatethrough pancreatic duct,and the rabbits in the control group were operated through the experimental group,but injected with equal volume distilled water.After 24h,the general condition of the rabbit's abdominal cavity was observed,the gastrointestinal transmission index(TI)was calculated,and the serum levels of GAS,MTL,SP,VIP,CCK,TNF-a,IL-1 and IL-6 were detected.The pathological sections of the pancreas were made,under the microscope,and the pathological score was observed.Results:In the experimental group,a large number of turbidity ascites,many saponification spots,large hemorrhage and necrosis of the pancreas,obvious hyperemia and edema of the gastrointestinal tract accompanied by partial necrosis were found in the abdominal cavity.The widening of the interleaf space of the pancreas tissue,the edema of the cells,the necrosis of some acinar cells and a large number of inflammatory cells infiltrated in the experimental group.The TI in the experimental group was lower than the control group,and PSP was significantly higher(P<0.05);The concentrations of GAS,SP and VIP in the experimental group were higher,and the concentration of MTL、CCK was lower(P<0.05).The concentrations of TNF-α,IL-1 and IL-6 in the experimental group were higher(P<0.05).Conclusion:SAP white rabbits have gastrointestinal motility disorders,which are closely related to the abnormal expression of gastrointestinal hormones and inflammatory factors.