利用大肠埃希氏菌光控基因表达系统降解多菌灵农残

Using Escherichia coli Light-regulated Gene Expression System to Degrade MBC

利用一种新型的重组大肠埃希氏菌光控基因诱导表达系统来生产多菌灵水解酶,用于环境中多菌灵农药残留的降解。该光控系统基于光敏融合蛋白LEV1在光照条件下发生同源二聚化并抑制下游基因转录的原理。SDS-PAGE和酶联免疫实验结果表明,异源多菌灵水解酶基因可通过光控机制被超表达,生产的多菌灵水解酶在溶液和土壤样品中具有降解多菌灵农药残留的活性。本技术利用光作为诱导剂,具备廉价易得、无毒害、可随时添加或去除等优势,是一种有前景并可大规模生产生物酶产品的工具。

A newly developed Escherichia coli light-regulated gene expression system was used to produce methyl-1h-benzimidazol- 2-yl carbamate(MBC)hydrolyzing esterase for degrading residual pesticide MBC in environment.This light-regulated system is based on dimerization of photosensitive protein LEV1 upon light exposure to repress the expression of downstream gene.SDS-PAGE and ELISA results showed that the gene of MBC hydrolyzing esterase could be overexpressed in our light-regulated system,by which the MBC hydrolyzing esterase was produced and it had enzymatic activity of degrading pesticide MBC in both liquid solution and soil samples.Light as an inducer,given its many advantages such as cheap,easy to be obtained,non-toxic,and added/removed at any time,is a promising tool for the industrial production of enzymes.