2型糖尿病骨质疏松患者血清脂肪细胞因子Apelin-13与骨密度的相关性

Correlation of pelin-13 and bone density in patients with type 2 diabetes

目的探讨2型糖尿病骨质疏松患者血清中脂肪细胞因子Apelin-13与骨密度的相关性。方法选取2016年8月至2017年8月西安医学院第二附属医院收治的2型糖尿病患者150例,根据世界卫生组织骨质疏松诊断标准分为骨量正常组、骨量减少组、骨质疏松组,每组各50例。糖化血红蛋白分析仪检测指尖血中糖化血红蛋白(Hb Alc)水平,酶联免疫吸附法检测血清中Apelin-13水平,放射免疫分析法检测血清中25-羟维生素D3[25(OH) D3]、1型原胶原N-端前肽(PINP)、1型胶原羧基端交联肽原(ICTP)水平。比较各组一般资料、Hb Alc、Apelin-13、PINP、ICTP、25(OH) D3水平及骨密度。对Apelin-13与PINP、ICTP、25(OH) D3水平及各检测位点骨密度进行相关性分析。结果骨质疏松组Apelin-13、25 (OH) D3水平最低,分别为(820. 44±73. 62) pg/m L、(7. 54±0. 95) nmol/L,骨量减少组次之,骨量正常组最高;骨质疏松组PINP、ICTP水平最高,分别为(1 332. 74±126. 45) pg/m L、(496. 32±55. 42)μg/L,骨量减少组次之,骨量正常组最低;组间比较差异均有统计学意义(P <0. 05)。相关性分析结果显示,血清Apelin-13水平与血清PINP、ICTP水平呈负相关(r=-0. 7234,-0. 8514,P <0. 05);与血清25(OH) D3水平呈正相关(r=0. 9533,P <0. 05);与各检测位点骨密度均呈正相关(P <0. 05)。结论 2型糖尿病骨质疏松患者血清Apelin-13、25(OH) D3水平较低,PINP、ICTP水平较高。Apelin-13可能参与了糖尿病患者的骨吸收和骨形成过程。

Objective To explore the correlation of Apelin-13 and bone density in patients with type 2 diabetes.Methods From Aug 2016 to Aug 2017,150 patients with type 2 diabetes were randomly selected from the Second Affiliated Hospital of Xi'an Medical University.According to WHO osteoporosis diagnostic criteria,normal bone mass group,bone mass reduction group and osteoporosis group,50 cases in each.The glycosylated hemoglobin analyzer detect HbAlc levels at the fingertips.ELISA was used to detect Apelin-13 levels in serum.Radioimmunoassay was used to the levels of 25(OH)D3,PINP,and ICTP in serum.The results of general data,HbAlc,Apelin-13,PINP,ICTP,25(OH)D3 and bone density were compared.The correlation between Apelin-13 and PINP,ICTP,25(OH)D3 level and the bone density of each test site were analyzed.Results The osteoporosis group Apelin-13,25(OH)D3,BMD level wthe lowest(820.44±73.62)pg/mL,(7.54±0.95)nmol/L,the bone mass reduction,and the normal bone mass group was the highest.The osteoporosis group had the highest level of PINP and ICTP(1 332.74±126.45)pg/mL,(496.32±55.42)μg/L,the lower bone mass group,and the normal bone mass group was the lowest(P<0.05).Correlation analysis showed that serum Apelin-13 level significantly negatively correlated with serum PINP and ICTP levels(r=-0.7234,-0.8514,P<0.05).There was a positive correlation with serum 25(OH)D3 level(r=0.9533,P<0.05).There was a significant positive correlation between the bone density(P<0.05).Conclusion Patients with type 2 diabetes with osteoporosis low serum Apelin-13,25(OH)D3 and high levels of PINP and ICTP.Apelin-13 may be involved in bone resorption and bone formation in diabetic patients.