电子束辐照对中华管鞭虾原肌球蛋白免疫原性及其构象的影响

Effect of Electron Beam Irradiation on Immunogenicity and Conformation of Tropomyosin from Shrimps(Solenocera melantho)

提取中华管鞭虾肉中的原肌球蛋白(tropomyosin,TM),采用不同电子束辐照剂量(0、1、3、5、7、9 kGy)处理,分别用免疫印迹法和间接酶联免疫吸附测定法检测TM与免疫球蛋白G(immunoglobulin G,IgG)的结合能力,采用圆二色光谱和荧光光谱法检测TM二级结构及其表面疏水性,探究电子束辐照引发的TM结构变化与免疫原性的关系。结果显示:电子束辐照会降低TM的含量;随辐照剂量的增加,TM分子中的α-螺旋含量降低,β-折叠和无规卷曲含量增加,分子表面疏水性增强,同时TM与IgG的结合能力下降。电子束辐照处理能改变中华管鞭虾TM的构象,从而降低其免疫原性。本研究结果为应用电子束辐照降低中华管鞭虾肉的潜在致敏性提供理论依据。

In order to illustrate the relationship between the conformation change of shrimp tropomyosin(TM)after electron beam(EB)irradiation and its immunogenicity,TM extracted from Solenocera melantho was irradiated with different EB doses(0,1,3,5,7 and 9 kGy).The secondary structure and surface hydrophobicity of the irradiated TM were analyzed by circular dichroism and fluorescence spectroscopy,and the immunoglobulin G(IgG)-binding capacity was determined using Western blot and indirect enzyme-linked immunosorbent assay(ELISA).The results showed that EB irradiation reduced the content of TM and changed its secondary structural composition of.Theα-helix content decreased,while theβ-sheet and random coil contents as well as the surface hydrophobicity increased with the increase of irradiation dose;meanwhile,the IgG-binding capacity decreased.In conclusion,it is demonstrated that EB irradiation could decrease TM immunogenicity by changing its conformational structure,which provides a theoretical basis for the application of EB irradiation to decrease the potential allergenicity of Solenocera melantho meat.