食用色素藻蓝蛋白对非小细胞肺癌A549细胞体外增殖和凋亡的影响

Effect of C-Phycocyanin as a Food Pigment on Proliferation and Apoptosis of Non-small Cell Lung Cancer A549 Cells

本研究以人类非小细胞肺癌A549细胞系为模型,采用藻蓝蛋白处理体外培养的细胞,分别从细胞存活率、细胞形态、细胞凋亡程度以及周期分布4个方面阐述了藻蓝蛋白对A549细胞的影响。结果表明,藻蓝蛋白能够显著降低A549细胞的存活率与生长速率(P<0.05),引起细胞形态发生非正常改变;同时上调促凋亡基因Bax、Bad,下调抑凋亡基因Bcl-2、Bcl-xl的表达而诱导细胞凋亡;此外,藻蓝蛋白能够异常调控细胞周期G1/S转换点相关基因的表达而引起细胞发生G1期阻滞。研究结果揭示了藻蓝蛋白对A549肺癌细胞具有显著的抑制作用,为开发新型抗癌类功能性食品着色剂提供了必要的理论依据。

The objective of this study was to investigate the effect of C-phycocyanin(C-PC)on the survival rate,morphology,apoptosis and cell cycle distribution of non-small cell lung cancer A549 cells.The results showed that C-PC could significantly reduce the survival rate and growth rate of A549 cells(P<0.05).It also could induce apoptosis by up-regulating the mRNA and protein expressions of the Bax and Bad genes and down-regulating the mRNA and protein expressions of the Bcl-2 and Bcl-xl genes.In addition,C-PC could regulate the expression of G1/S check point-related genes,thereby causing G1 phase arrest.These results indicated C-PC had a significant inhibitory effect on A549 cells,which could provide the essential theoretical basis for the development of new anti-cancer food colorants.