pH对日本囊对虾的急性毒性初步研究

Preliminary study on acute toxicity of pH to Marsupenaeus japonicus

采用静水试验方法,研究低pH对日本囊对虾(Marsupenaeus japonicus)的急性毒性。试验用日本囊对虾平均体长(48. 87±4. 81) mm,设定pH梯度为3. 8、4. 1、4. 4、4. 7、5. 0,对照组pH为8. 0±0. 1,统计日本囊对虾在低pH胁迫下的死亡率,分析pH对日本囊对虾的半致死摩尔浓度(LC50)和半致死时间(LT50)。结果显示:在相同pH下,日本囊对虾的死亡率随时间延长而增加;在相同的胁迫时间内,日本囊对虾死亡情况与水体pH呈负相关(R <0,P <0. 01); pH对日本囊对虾胁迫48 h、72 h、96 h的半致死摩尔浓度(LC50)分别为:7. 907×10^(-5)、5. 140×10^(-5)和3. 673×10^(-5)mol/L,安全摩尔浓度(SC)为3. 673×10-6mol/L,对应的pH分别为4. 102、4. 289、4. 435、5. 435;水体pH 4. 1、pH 4. 4、pH 4. 7胁迫日本囊对虾的半致死时间(LT50)分别为58. 821、93. 771和139. 549 h。本研究可为研究日本囊对虾的养殖、对低pH的耐受性及耐低pH品系选育提供参考。

Acute toxicity of low pH to Marsupenaeus japonicus was studied by hydrostatic experiment.We selected Marsupenaeus japonicus with an average length of(48.87±4.81)mm as experimental subjects,and set pH gradient as Marsupenaeus japonicus 3.8,4.1,4.4,4.7,5.0 and pH of control group as 8.0±0.1 to investigate the mortality of Marsupenaeus japonicus under low pH stress and analyze the semi-lethal concentration(LC50)and semi-lethal time(LT50)of Marsupenaeus japonicus under pH stress.The results showed that:the mortality of Marsupenaeus japonicus increased with time under the same pH.There was a negative correlation between the mortality of Marsupenaeus japonicus and the pH in water(R<0,P<0.01)at the same stress time.The LC50 of Marsupenaeus japonicus under stress of 48 h,72 h,96 h with pH was 7.907×10^-5,5.140×10^-5 and 3.673×10^-5 mol/L,and safe concentration(SC)was 3.673×10^-6 mol/L,corresponding pH was 4.102,4.289,4.435 and 5.435 respectively.The LT50 of Marsupenaeus japonicus under water pH=4.1,4.4,4.7 was 58.821,93.771,139.549 h respectively.This study provided a basis for studying the breeding of Marsupenaeus japonicus,the tolerance to low pH and the selection of low pH resistant strains.