白藜芦醇对大鼠脑缺血再灌注海马损伤的保护作用及对MMP-9表达的影响

Protective effects of resveratrol on hippocampus damage and its influence on MMP-9 expression in ischemia-reperfusion rats

目的研究白藜芦醇(RES)对大鼠脑缺血再灌注(I/R)损伤的保护作用及对基质金属蛋白酶-9(MMP-9)表达的影响。方法将大鼠分为假手术组(Sham组)、I/R组和RES组,每组8只。采用线栓法建立短暂性右侧大脑中动脉闭塞模型,造模前连续10d给予药物干预,I/R后进行神经功能损害评价,并检测大脑中动脉血流量变化,最后应用免疫组织化学技术检测海马MMP-9表达。结果 I/R组和RES组均表现出左侧肢体肌力降低,与Sham组相比差异有统计学意义(P<0.05)。与Sham组相比,I/R组和RES组大鼠均表现大脑中动脉血流量不同程度降低(P<0.05)。Sham组的海马CA1和CA2区MMP-9的平均光密度值均明显低于I/R组和RES组(P<0.01),I/R组明显高于RES组(P=0.011)。结论 I/R前预防性给予RES能有效改善大鼠神经功能损伤预后,其机制可能与RES抑制MMP-9的活性表达有关。

Objective To study the protective effect of resveratrol(RES)on the hippocampus damage and its influence on expression of MMP-9 in ischemia-reperfusion(I/R)rats.Methods The rats were divided into the sham operation group(Sham group),I/R group and RES group,8 cases in each group.The transient right side middle cerebral artery occlusion model was established by adopting the suture-occluded method.The medication intervention was given for continuous 10 d before constructing the model.The damage of nerve function was evaluated after I/R and the change of middle cerebral artery blood flow was detected.Finally,the immunohistochemistry technique was adopted to measure the expressive level of MMP-9 in hippocampus.Results The left limb muscle strength in the I/R group and RES group was decreased,which showed the statistical difference compared with the Sham group(P<0.05).Compared with the Sham group,the I/R group and RES group manifested by different degrees of cerebral blood flow decrease(P<0.05).The average optical density value level of MMP-9 in the hippocampus CAI and CA2 areas in the Sham group was significantly lower than that in the I/R group and RES group(P<0.01),while the I/R group was significantly higher than the RES group(P=0.011).Conclusion Prophylactically use of RES could effectively improve the prognosis in rat neural function damage and its mechanism might be related with RES inhibiting the MMP-9 active expression.