花生荚壳抗黄曲霉菌侵染的鉴定方法研究及抗性种质发掘

Method for screening resistance of peanut shell to Aspergillus flavus infection and identification of resistant genotypes

黄曲霉毒素污染是影响花生食用安全性和制约产业发展的重要因素。荚壳是花生抵御黄曲霉菌侵染的第一道防线,为建立花生荚壳抗黄曲霉侵染的鉴定方法,本研究利用强侵染黄曲霉菌AF2202接种花生荚果,通过对不同接种菌浓度和培养时间的比较分析,发现接种浓度为2×10~6孢子/mL、培养7d的组合方案可以有效区分花生荚壳对黄曲霉菌侵染的抗性。利用所建立的鉴定方法对276份遗传变异丰富的花生核心种质材料进行接种鉴定,进一步证明了这一方法鉴定花生荚壳抗性的有效性和实用性,初步发掘出2份具有荚壳抗性的特异花生种质。

Aflatoxin contamination has been a key factor affecting food safety of peanut products and a serious constraint to peanut industry.The resistance to infection of Aspergillus flavus in shell is the preliminary physical protection mechanism for reducing risk of toxin contamination in peanut.However,very limited research has been done on identification method for shell resistance to A.flavus infection.In this research,peanut pods were inoculated with A.flavus strain AF2202 with 5 concentrations of inoculum solutions and then incubated for 3-9 days in darkness.Through comparing various combinations of inoculation and incubation period,the optimal combination for identifying shell resistance was 2 × 10^6 spores per milliliter and 7 days.A total of 276 diversified lines consisting of peanut core collections were tested using the newly-developed method,and the results further confirmed that the method was effective and practicable.Significant difference in shell resistance was found among the involved germplasm lines and 2 elite lines with shell resistance were identified.