澳洲茶树离体培养器官发生的形态解剖学观察

Morphological and Anatomical Observation of Organogenesis from Melaleuca alternifolia in vitro Culture

采用石蜡切片和光学显微技术对澳洲茶树(Melaleuca alternifolia)组培苗离体器官发生进行形态解剖学观察。结果表明:在选定的培养基上,澳洲茶树芽的增殖直接由器官诱导形成,有效地保持了其遗传的稳定性。在诱导生根前,没有潜在的根原基,说明其生根类型为诱导生根型;不定根发生形式属皮部生根类型;但生根过程中诱导产生愈伤组织,其对根原基的形成有一定刺激作用,因此,诱导出适当的愈伤组织,对澳洲茶树不定根的生成具有重要的作用。

The morphological and anatomical observation on the organogenesis from tissue-culture plantlets of Melaleuca alternifolia was carried out with the method of paraffin section and optical microscopy technique.The results were obtained as follows:in the selected culture medium,the multiplied buds of M.alternifolia formed directly through the organogenesis way,thus the genetic stability of this plant could be effectively maintained.There was no potential root primordium before the rooting was induced,suggesting that its rooting type belonged to induced type.The type of adventitious root organogenesis belonged to cortex-rooting type.But callus was induced out during the rooting,which could stimulate the formation of root primordium.Therefore,inducing proper quantity of callus played an important role in the formation of adventitious roots of M.alternifolia in vitro culture.