痰液CNRIP1基因甲基化检测对于肺癌诊断的意义

Significance of CNRIP1 gene methylation in sputum in diagnosis of lung cancer

目的研究肺癌患者的肿瘤组织及痰液标本麻素受体相互作用蛋白-1(cannabinoid receptor interacting protein 1,CNRIP1)基因及其甲基化,分析讨论痰液基因甲基化对于肺癌早期诊断的意义。方法通过甲基化特异性PCR(MSP)法对于80例肺癌患者的肺癌组织、癌旁组织及痰液标本和30例健康对照者的痰液标本进行基因甲基化检测。并且分析痰液CNRIP1基因甲基化发生与患者的性别、吸烟状况、病理分型、病理分期之间的相关性。结果肺癌组织CNRIP1基因甲基化发生率为85%,而癌旁组织其甲基化发生率为8. 75%,差异具有统计学意义(P <0. 01);痰液标本中肺癌患者痰液CNRIP1基因甲基化发生率为78. 75%,健康对照组痰液基因甲基化发生率仅为3. 33%,差异具有统计学意义(P <0. 01)。痰液CNRIP1基因甲基化状态与性别、吸烟状况、病理分期等无相关性,但是,在鳞癌患者外周血标本中,其甲基化发生率(91. 1%)较其他类型肺癌患者明显增高,差异具有统计学意义(P=0. 009)。结论痰液CNRIP1基因甲基化检测对于肺癌的早期诊断具有较高的敏感度和特异度,并且对于肺鳞癌的诊断具有更高的敏感度,对于肺癌的病理分型具有一定的理论指导意义。

Objective To investigate the expression of cannabinoid receptor interacting protein 1(CNRIP1)gene in tissues and sputum samples from patients with lung cancer,and explore the significance of gene methylation in sputum for the early diagnosis of lung cancer.Methods Methylation specific PCR(MSP)method was used to detect gene methylation in the lung cancer tissues,adjacent tissues and sputum samples of 80 lung cancer patients and 30 healthy sputum samples.The relationships between the methylation of CNRIP1 gene in sputum and sex,smoking status,pathological type and staging of the patients were analyzed.Results CNRIP1 gene methylation rate was 85%in lung cancer tissues and 8.75%in paracancerous tissue.CNRIP1 gene methylation occurred in 78.75%of sputum samples of lung cancer and 3.33%of the sputum samples of healthy controls.There was no relationship between the methylation status of CNRIP1 gene in sputum and sex,smoking status and pathological stage.However,the methylation rate in sputum of patients with squamous carcinoma(91.1%)was significantly higher than that in other types of lung cancer patients,and the difference was statistically significant(P=0.009).Conclusion Detection of CNRIP1 gene methylation in sputum has high sensitivity and specificity in the early diagnosis of lung cancer,and has higher sensitivity in the diagnosis of lung adenocarcinoma.It has certain theoretical guiding significance for pathological classification of lung cancer.