三个多倍体萱草品种的组培快繁

Tissue Culture and Rapid Propagation of Three Cultivars of Polyploid Hemerocallis fulva

以多倍体萱草三个品种(代号分别为28、41、42)的花茎及幼蕾为外植体进行组培研究,结果表明,花茎的愈伤诱导及其相应的芽分化明显好于花蕾,花茎做外植体更适宜;以MS+(2.0～2.5) mg/L BA+(0.20～0.25) mg/L NAA培养基的愈伤诱导效果较好,三个品种中以41号愈伤形成早且量大,28号次之,42号愈伤出现较晚且量较少。在诱导芽分化时,41号和28号在MS+2.5 mg/L BA+(0.20～0.25) mg/L NAA培养基上芽分化数量多,41号最易芽分化,28号次之;42号需要多代的芽诱导驯化培养才分化出芽,在MS+(2.5～3.0) mg/L BA+(0.25～0.30) mg/L NAA培养基上分化数量较多,但分化率低于41号和28号。三个品种在MS+2.5 mg/L BA+0.25 mg/L NAA培养基上的增殖效果较好,在1/2MS+0.10 mg/L NAA中适宜生根培养。

Three cultivars of polyploid Hemerocallis fulva (cultivar number 28, 41 and 42) were selected for tissue culture by using their floral axis and flower buds as explants. The results were as follows: the callus produced from floral axis were much more and the buds were more easily differentiated compared with flower bud, so the floral axis was preferably as explants. The culture media of MS+(2.0~2.5) mg/L BA+(0.20~ 0.25) mg/L NAA were better for callus induction. The callus induced from cultivar 41 was the earliest and the most followed by the cultivar 28, while that from the cultivar 42 was the latest and least. In inducing buds, the media of MS+2.5 mg/L BA+(0.20~0.25) mg/L NAA induced much more buds from the cultivars 41 and 28,in which, it was more easily to induce buds from the cultivar 41 compared with 28. It needed for many generations of domestication culture to induce buds for the cultivar 42, and the media of MS+(2.5~ 3.0) mg/L BA+(0.25~0.30) mg/L NAA was optimal, but its inducing rate was lower than that of the cultivars 41 and 28. The medium of MS+2.5 mg/L BA+0.25 mg/L NAA was better for multiplication culture, and the medium 1/2MS+0.10 mg/L NAA was appropriate for rooting culture.