化瘀解毒中药抑制梗阻性肾病巨噬细胞浸润减轻炎性损伤的作用

Inhibitory effects of Huayu Jiedu decoction on macrophage infiltration to alleviate infalmmatory injury in obstructive nephropathy rats

为观察化瘀解毒中药对梗阻性肾病巨噬细胞浸润的影响及作用机制。将48只健康雄性Wistar大鼠随机分为假手术组、模型组、依普利酮组、中药组,每组12只。除假手术组外,其余大鼠结扎单侧输尿管(UUO)复制肾间质纤维化动物模型。治疗组分别给以依普利酮(100 mg/kg/d加入饲料喂养)和化瘀解毒中药煎剂(14 g/kg/d灌胃)。10 d后摘取肾脏,观察大鼠肾脏组织病理改变。免疫组化法标记巨噬细胞浸润,免疫组化、Western Blot方法检测血清和糖皮质激素诱导蛋白激酶1(SGK-1)、单核细胞趋化蛋白-1(MCP-1)、白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)的表达。肾脏病理显示,UUO组大鼠肾脏有明显的肾小管扩张及上皮细胞脱落,间质巨噬细胞浸润增多和细胞外基质(ECM)大量积聚,SGK-1、MCP-1、IL-1、TNF-α表达明显增强。化瘀解毒中药可明显减轻UUO大鼠肾脏巨噬细胞等炎性细胞浸润和ECM沉积,下调SGK-1、MCP-1、IL-1、TNF-α表达。以上结果说明化瘀解毒中药可抑制梗阻性肾病诱导的巨噬细胞浸润,减轻肾脏炎性损伤。

To observe the effect of Huayu Jiedu decoction on macrophages infiltration in obstructive nephropathy rats,48 male Wistar rats were randomly divided into sham group,UUO group,EPL group and ZY group(n=12).Except the rats in sham group,the rats in the other groups were prepared unilateral ureteral obstruction(UUO)for renal interstitial fibrosis model.The rats were treated with 14 g/kg/d decoction of Chinese medicine in ZY group orally,and eplerenone at 100 mg/kg/d added to diet in EPL group.The kidneys were harvested to observe the histopathological changes at 10th day after surgery.Macrophage infiltration marked with F4/80 was examined by immunohistochemistry.The Serum and glucocorticoid induced kinase-1(SGK-1),Monocyte chemotactic protein-1(MCP-1),interleukin-1(IL-1)and tumor necrosis factor-α(TNF-α)were detected with immunohistochemistry and western blot.Renal pathology showed that renal tubular dilatation and epithelial cell shedding in UUO rats significantly.The infiltrated macrophage and accumulated extracellular matrix(ECM)also increased in interstitium of UUO rats markedly.The macrophages infiltration and ECM accumulation were attenuated by treated with Chinese herb decoction and eplereneone.Expressions of SGK-1,MCP-1,IL-1 and TNF-αwere up-regulated in the UUO rats and were suppressed by Chinese herb decoction.Chinese herb decoction could inhibit the inflammatory injury via reducing macrophage infiltration in UUO rats.