雪松松针多糖超声波酶法提取及其抗氧化性

Ultrasonic-assisted enzymatic extraction and antioxidant activity of polysaccharide from Cedrus deodara pine needles

为优化雪松松针多糖超声波酶法的提取工艺,并研究多糖结构及其抗氧化性。通过响应面法分析确定最佳提取参数为:3. 0 g松针粉末,液料比20∶1(m L∶g),提取温度80℃,超声功率560 W,超声时间47 min,纤维素酶用量12 FPU/g原料,提取两次,多糖得率高达10. 39%。采用高效液相色谱、红外光谱和核磁共振光谱等对松针多糖进行了结构表征,松针多糖以β-糖苷键为主要连接方式,并由葡萄糖、果糖、阿拉伯糖和半乳糖等单糖组成。体外抗氧化性研究结果表明:松针粗多糖对羟基自由基(·OH)和1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除能力远高于纯化多糖,呈现出良好的量效关系,粗多糖对·OH和DPPH·的半抑制浓度IC50分别为0. 47 g/L和0. 076 g/L。

In order to optimize the ultrasonic-assisted enzymatic extraction process of polysaccharide from Cedrus deodara pine needles,and investigate the structure of polysaccharide and its antioxidant activity.The optimal extraction parameters were determined by response surface methodology as follows:3.0 g pine needle powder,liquid to material ratio 20∶1(mL∶g),extraction temperature 80℃,ultrasonic power 560 W,ultrasonic extraction time 47 min with the amount of cellulase 12 FPU/g raw material,and extraction twice,the polysaccharide yield was as high as 10.39%.The structure of pine needle polysaccharide was characterized by high performance liquid chromatography,infrared spectroscopy and nuclear magnetic resonance spectroscopy.The purified polysaccharide,linked mainly by β-glycosidic bonds,consisted of monosaccharides such as glucose,fructose,arabinose and galactose.Antioxidant activities of the polysaccharides were evaluated on the basis of hydroxyl radical(·OH)and 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·).The scavenging ability of the crude polysaccharide was much higher than that of purified polysaccharide,which showed a good dose-effect relationship.The semi-inhibitory concentrations IC50 of the crude polysaccharide on·OH and DPPH·were 0.47 g/L and 0.076 g/L,respectively.