离子色谱-脉冲安培法测定芦荟多糖中7种单糖的含量

Determination of seven monosaccharides in aloe polysaccharide using ion chromatography with pulsed amperometric detector

采用水提醇沉法提取芦荟鲜叶中的多糖,经三氟乙酸水解,利用Dionex CarboPac PA10高效阴离子色谱柱分离,氢氧化钠梯度淋洗,积分脉冲安培检测,建立了芦荟多糖中岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖和木糖7种常见单糖的测定方法。结果表明:7种单糖在线性范围内的相关系数(R^2)均高于0.997,检出限为0.007～0.024 mg/L,加标回收率为97.5%～102.5%,相对标准偏差(RSD)为0.1%～4.8%。该法简单、快速、灵敏、准确,可用于芦荟多糖中单糖含量的测定和多糖组成的研究。

Aloe polysaccharide was extracted by water extraction and ethanol precipitation.It was hydrolyzed by trifluoroacetic acid,eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column,and detected using a pulsed amperometric detector.This method was used to determine the fucose,rhamnose,arabinose,galactose,glucose,mannose,and xylose in aloe polysaccharide.The results showed that the correlation coefficients(R^2)of all the seven target compounds were more than 0.997 in the linear range,and the limits of detection were between 0.007 mg/L and 0.024 mg/L.The recoveries were in the range 97.5%-102.5%with the relative standard deviations in the range 0.1%-4.8%.The method is simple,rapid,sensitive,and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.