中缅边境流行区恶性疟原虫野生株驯化方式的研究

STUDY ON THE DOMESTICATION OF PLASMODIUM FALCIPARUM WILD ISOLATES FROM CHINA-MYANMAR BORDER ENDEMIC AREA

本文旨在探讨恶性疟原虫野生株体外培养的驯化方法。在中缅边境流行区采集12株野生型恶性疟原虫样本,采用添加ALBUMAX II、次黄嘌呤的改进配方,通过逐渐脱离灭活去纤维蛋白原人血浆的培养方式进行人工驯化临床恶性疟原虫株。分别对灭活去纤维蛋白原人血浆对野生型虫株驯化过程及驯化后生长的影响做统计学分析。结果表明灭活去纤维蛋白原人血浆对野生株体外驯化过程生长的影响具有统计学意义,对人工驯化后生长影响不具有统计学意义。最终N13-1231和F08B38两虫株经驯化后可脱离灭活去纤维蛋白原人血浆连续培养。本文成功对中缅边境流行区恶性疟原虫野生株N13-1231和F0838进行驯化,可脱离灭活去纤维蛋白原人血浆连续培养,具有科学研究价值。

Attempts to domesticate wild isolates of Plasmodium falciparum in vitro were conducted in this study. Total 12 P. falciparum isolates were collected from malaria epidemic areas along the China - Myanmar border. With the modified culture medium by adding ALBUMAX II and hypoxanthine, the clinical P. falciparum isolates were gradually domesticated and cultured without defibrinated heat - inactivated human plasma, which benefit the growth of wild isolates significantly. After domestication, the P. falciparum isolates N13 -1231 and F08B38 were shown to propagate continuously in the culture medium without defibrinated heat - inactivated human plasma. The successful domestication of P. falciparum isolates on the culture medium without defibrinated heat - inactivated human plasma gave a limestone for further researches.