脂联素对高糖条件下视网膜血管内皮细胞增殖、迁移和管腔形成的作用

Effects of adiponectin on proliferation,migration and tube formation of retinal vascular endothelial cells under high glucose conditions

目的:观察脂联素(APN)对高糖培养条件下恒河猴脉络膜视网膜血管内皮细胞(RF/6A细胞)增殖、迁移和管腔形成的影响。方法:将体外培养的RF/6A细胞随机分为空白对照组(正常培养基培养)、甘露醇对照组(含20mmol/L甘露醇的培养基培养)、高糖组(含25mmol/L D-葡萄糖的培养基培养)和高糖+APN组(含25mmol/L D-葡萄糖+10μg/mL APN的培养基培养)。分别采用CCK-8法检测细胞增殖,Transwell小室检测细胞迁移,基质胶(Matrigel)法检测细胞管腔形成能力。结果:空白对照组与甘露醇对照组细胞增殖率(100.00%±0.00%vs 99.09%±0.46%)、迁移数(121.60±6.02个vs 119.60±9.39个)及管腔形成数(12.11±0.84个vs 12.22±0.96个)均无差异(P>0.05)。高糖组细胞增殖率(71.42%±2.29%)明显低于空白对照组和甘露醇对照组,细胞迁移数(144.20±9.65个)和管腔形成数(16.00±2.90个)均明显高于空白对照组和甘露醇对照组(P<0.05)。高糖+APN组细胞增殖率(90.87%±1.68%)明显低于空白对照组和甘露醇对照组,明显高于高糖组;细胞迁移数(73.00±6.04个)和管腔形成数(7.89±0.38个)均明显低于空白对照组、甘露醇对照组及高糖组(P<0.05)。结论:APN可以促进高糖条件下RF/6A细胞存活及增殖,抑制细胞迁移和管腔形成,提示APN对高糖导致的RF/6A细胞损伤具有一定的保护作用,抑制病理刺激条件下的血管生成。

·AIM:To observe the effect of adiponectin(APN)on proliferation,migration and tube formation of RF/6 A(monkey choroid and retinal endothelial cell line)cells cultured in the conditions of high glucose.·METHODS:Well-cultured RF/6 A cells in vitro were randomly divided into four groups:blank control group,mannitol control group,high glucose group and high glucose+10μg/mL APN group.Cell proliferation,migration and tube formation was detected by CCK-8 assay,transwell chamber and matrigel assay,respectively.·RESULTS:There was no significant difference in the cell proliferation rate(100.00%±0.00%vs 99.09%±0.46%),the number of cell migration(121.60±6.02 vs 119.60±9.39)and the number of tube formation(12.11±0.84 vs 12.22±0.96)between the blank control group and mannitol control group(all P>0.05).The cell proliferation rate of the high glucose group(71.42%±2.29%)was significantly lower than that of the blank control group and the mannitol control group,and the number of cell migration(144.20±9.65)and tube formation(16.00±2.90)of the high glucose group were significantly higher than that of the blank control group and the mannitol control group(all P<0.05).The proliferation rate of cells in the high glucose+APN group(90.87%±1.68%)was significantly lower than that of the blank control group and the mannitol control group,while higher than that in the high glucose group(all P<0.05).The number of cell migration(73.00±6.04)and tube formation(7.89±0.38)in the high glucose+APN group was significantly lower than that of the blank control group,the mannitol control group and the high glucose group(all P<0.05).·CONCLUSION:APN can promote the survival and proliferation of RF/6 A cells,and inhibit the migration and tube formation of RF/6 A cells under high glucose conditions,suggesting that APN has a protective effect on the damage of RF/6 A cells caused by high glucose and inhibits the angiogenesis process under pathological stimulations.