獐宝对大鼠胃黏膜HSP60、HSP90、Cyt-c、Caspase9表达的影响

Effect of Zhangbao on the Expression of HSP60, HSP90, Cyt-c and Caspase9 in Gastric Mucosa of Rat

目的探讨獐宝治疗胃癌癌前病变的机理。方法清洁级Wistar雄性大鼠14只,随机分为4组,空白组(N),模型对照组(NR),獐宝治疗组(D),胃复春对照组(W),每组3只,剩余2只16周时取样观察造模情况。12周后处死大鼠,剖腹取胃,应用荧光定量PCR及免疫印迹法检测各组胃组织样本中HSP60、HSP90、Cyt-c、Caspase9蛋白的表达。结果胃组织荧光定量PCR及免疫印迹法均显示:与空白组相比,模型对照组的HSP60、HSP90的表达减少(P<0.05);而与模型对照组相比,给药组HSP60、HSP90的mR NA表达均有所增加(P<0.05),其中獐宝组较胃复春组增加更明显(P<0.05)。与空白组相比,模型对照组的Cleaved-Caspase9、Cyt-c的mRNA表达增加(P<0.05),而与模型对照组相比,给药组Cleaved-Caspase9、Cyt-c的mRNA表达有所减少,其中獐宝组较胃复春组减少更明显(P<0.05)。结论胃癌癌前病变的机制可能与凋亡信号通路过度激活有关,獐宝与胃复春能够抑制凋亡通路的过度激活,其中獐宝疗效优于胃复春。

Objective To investigate the mechanism of Zhangbao in the treatment of precancerous lesions of gastric cancer.Methods Fourteen Wistar male rats were randomly divided into 4 groups:blank group(N),model control group(NR),Zhangbao treatment group(D),and Weifuchun control group(W).Only the remaining 2 samples were taken at 16 weeks to observe the modeling.After 12 weeks,the rats were sacrificed and the stomach was taken by laparotomy.The expressions of HSP60,HSP90,Cyt-c and Caspase9 in gastric tissue samples were detected by real-time PCR and immunoblotting.Results Quantitative PCR and immunoblotting of gastric tissue showed that the expression of HSP60 and HSP90 in the model control group was lower than that in the blank group(P<0.05).Compared with the model control group,the administration group was HSP60 and HSP90.The mRNA expression was increased(P<0.05),and the Zhangbao group was more significantly increased than the Weifuchun group(P<0.05).Compared with the blank group,the mRNA expression of Cleaved-Caspase9 and Cyt-c in the model control group was increased(P<0.05),while the mRNA expression of Cleaved-Caspase9 and Cyt-c in the drug-administered group was compared with the model control group.The reduction was more obvious in theZhangbao group than in the Weifuchun group(P<0.05).Conclusion The mechanism of precancerous lesions of gastric cancer may be related to the excessive activation of apoptotic signaling pathway.Zhangbao and Weifuchun can inhibit the excessive activation of apoptotic pathway,and the efficacy of Zhangbao is better than that of Weifuchun.