瘦素对人甲状腺乳头状癌K1细胞增殖、迁移及侵袭的影响

Regulation of Leptin on the Proliferation,Migration and Invasion of Human Papillary Thyroid Cancer K1 Cells

目的探讨不同浓度的瘦素对人甲状腺乳头状癌K1细胞增殖、迁移、侵袭能力的影响。方法分别用0ng/ml(空白对照组)、10ng/ml、50ng/ml、100ng/ml、125ng/ml,5组不同浓度的瘦素处理人甲状腺乳头状癌K1细胞,MTT实验检测不同浓度瘦素处理后细胞的增殖能力、划痕实验检测细胞迁移能力、Transwell实验检测细胞侵袭能力。结果 MTT实验检测结果显示,瘦素对K1细胞的增殖没有影响(P> 0. 05);不同浓度的瘦素处理K1细胞后24h后划痕实验迁移率分别为:40. 201%、47. 383%、59. 950%、68. 732%、79. 306%; 48h后划痕实验迁移率分别为:49. 398%、74. 192%、86. 733%、87. 795%、90. 005%。随瘦素浓度增加与处理时间延长,划痕内面面积缩小,迁移率增加(P <0. 05)。Transwell实验结果显示,穿出聚碳酸酯膜的细胞数依次增多(P <0. 05)。结论随处理时间的延长和瘦素浓度的增加,人甲状腺乳头状癌K1细胞增殖能力不受影响,K1细胞迁移和侵袭能力均呈上升趋势。

Objective To study the effect of different concentration of leptin on the proliferation,migration and invasion on human papillary thyroid cancer K1 cells.Methods Different concentrations of leptin(0ng/ml,10ng/ml,50ng/ml,100ng/ml,125ng/ml)were used to treat papillary thyroid cancer K1 cells,and the MTT assay,scratch assay and transwell were used to assess the viability and invasive ability of K1 cells respectively.Results The MTT assay results that leptin had no effect on the proliferation K1 cells(P>0.05).K1 cells were treated different concentration of leptin.After 24 hours the scratch test migration rates were:40.201%,47.383%,59.950%,68.732%,79.306%.48 hours of scratch test migration rates were:49.398%,74.192%,86.733%,87.795%,90.005%.The results that the increase of leptin concentration and the extension of treatment time area were reduced and mobility enhanced.Transwell show that the number of cells traversing in polycarbonate membrance increasing trend sequentially(P<0.05).Conclusion Leptin had no effect on the proliferation of K1 cells.But it could promote migration and invasion on K1 cells.