嗜水气单胞菌感染青鱼肝脏的蛋白质组学分析

QUANTITATIVE PROTEOMICS ANALYSIS OF LIVER OF BLACK CARP MYLOPHARYNGODON PICEUS UNDER AEROMONAS HYDROPHILA INFECTION

采用同重同位素标记相对与绝对定量(iTRAQ)结合液相色谱串联质谱(LC-MS/MS)技术,分析嗜水气单胞菌(Aeromonas hydrophila)感染青鱼(Mylopharyngodon piceus)后青鱼肝脏组织的差异表达蛋白。以致病性嗜水气单胞菌菌株BCK0712注射感染健康青鱼,24h后采集感染组和对照组青鱼肝脏,开展蛋白质组学分析。通过数据库检索,共鉴定到4475个肝脏组织蛋白,从中筛选到188个差异表达蛋白,其中表达上调的蛋白70个,表达下调的蛋白118个。经生物信息学分析,表明这些差异表达蛋白主要参与了补体和凝血级联反应、剪接体、细胞内吞作用、氧化磷酸化、碳代谢、精氨酸和脯氨酸代谢、色氨酸代谢等通路。组织病理分析表明,青鱼感染嗜水气单胞菌后肝脏出现了明显的病理变化,主要表现为肝细胞边界不分明、细胞核固缩、肝板排列紊乱、有出血现象等。研究结果为进一步深入探究嗜水气单胞菌的致病机制提供了理论基础。

To investigate whether Aeromonas hydrophila generally mediate liver protein expression,isobaric tags for relative and absolute quantitation(iTRAQ)combined with LC-MS/MS were used to screen liver proteins of black carp Mylopharyngodon piceus after infected with Aeromonas hydrophila(BCK0712)for 24 hours.Differentially expressed proteins were screened through identification and quantification analysis.As a result,a total of 4475 proteins were identified and 188 differentially expressed proteins were quantified upon bacterial infection,including 70 up-regulated and 118 down-regulated proteins.Bioinformatics analysis indicated that these differentially expressed proteins were mainly associated with complement and coagulation cascades,spliceosome,endocytosis,oxidative phosphorylation,carbon metabolism,arginine and proline metabolism,etc.Hispathological characteristics including unclear cell boundary,hemorrhage at different degrees and necrosis were observed on liver of black carp infected with A.hydrophila under microscope after hematoxylin-eosin staining.The potential mechanism of regulated proteins by A.hydrophila infection in liver pathology need further investigation.