聚己内酯/壳聚糖核壳结构纤维引导组织再生膜的制备及表征

Preparation and characterization of a guided tissue regeneration membrane constructed by core-shell polycaprolactone/chitosan fibers

高性能的引导组织再生膜是牙周引导组织再生术成功的关键,静电纺丝法因可仿生制备类细胞外基质结构,在引导组织再生膜研制方面显示出巨大潜力。本研究通过同轴静电纺丝法,以聚己内酯(PCL)为核层,壳聚糖(CS)为壳层,制备核壳结构的纳米纤维,并用香草醛对制备的纤维膜进行交联。利用扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FTIR)、力学测试及细胞培养等手段对制备的纤维膜进行形貌、内部结构、化学组成、力学性能和细胞相容性表征。结构分析表明本研究成功制备了核壳结构的PCL-CS纤维膜。力学测试和亲疏水性测试结果表明交联后的纤维膜具有较好的耐水性和力学性能,断裂强度高出文献报道值近两倍;体外细胞培养结果显示MG-63细胞能在交联后的纤维膜上黏附和持续增殖,表明纤维膜具有较好的细胞相容性,在引导组织再生领域有较好的应用前景。

High-performance guided tissue regeneration (GTR) membrane is a key factor for successful GTR treatment. Electrospinning has great potential for construction of a GTR membrane because this technique can prepare fibrous structural membrane similar to extracellular matrix, which is beneficial for cell adhesion and proliferation. In this study, the core-shell nanofibers were prepared by coaxial electrospinning technique with polycaprolactone (PCL) as the core and chitosan (CS) as the shell, and the resulting membrane was crosslinked by vanillin. Furthermore, the morphology, internal structure, chemical composition, mechanical properties and cytocompatibility of the fabricated membrane were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), X ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), mechanical testing and cell culture experiments. Structure analysis showed a core-shell PCL-CS nanofiber constructed membrane was successfully prepared. The results of mechanical and contact angle test showed that the crosslinked fiber membrane had better water resistance, higher mechanical properties and a tensile strength nearly two times higher than the reported studies. Cell culture results showed that the crosslinked fibrous membrane was a good candidate for MG-63 cell adhesion and proliferation, indicating the fabricated membrane has good cytocompatibility. In general, the fibrous GTR membrane constructed by core-shell PCL-CS nanofibers holds promising application.