高效DMF降解菌群的降解特性及影响因素

Degradation characteristics and influencing factors of DMF degrading communities

从浙江省某皮革厂生化池内的活性污泥中培养得到一组高效利用二甲基甲酰胺(DMF)作为碳源和氮源生长的混合菌群。经群落结构鉴定,菌群主要包含Brevundimonas sp.、Flavobacterium sp.、Nocardioidaceae sp.、Brevibacillus parabrevis和Stenotrophomonas sp.等。批式试验表明,DMF降解菌群的最佳接种率为30%,在pH为5.0～9.0的范围内均有氧化效果,最适pH为6.0,在48h内可将培养基中0.2%的DMF完全分解。菌群对DMF的最大耐受体积分数为6%,体系中氨氮浓度超过2000mg/L时,菌群仍具备DMF分解能力,硝酸盐浓度低于450mg/L时对降解效果影响较小。经7天驯化,菌群还可具备对同系物二甲基乙酰胺(DMAC)的分解能力。

Bacterial communities were obtained from activated sludge of a tannery in Zhejiang province, which grew by utilizing DMF(N,N-dimethylformamide) as carbon and nitrogen source. The community structure indicated that the microflora mainly consisted of: Brevundimonas sp., Flavobacterium sp., Nocardioidaceae sp., Brevibacillus parabrevis and Stenotrophomonas sp..The optimal inoculation rate was 30% and in the range of pH was 5.0-9.0, the microflora showed the oxidation effect and 0.2% of DMF in the medium could be completely decomposed within 48 hours when the optimal pH was 6.0. The maximal tolerable concentration of DMF was 6%. The concentration of ammonia nitrogen in the system exceeded 2000mg/L, the strains still had the ability to decompose DMF, and the nitrate concentration lower than 450mg / L had little effect on the degradation effect. After 7 days of acclimation, the bacteria could decompose DMAC (dimethylacetamide).