摘要
目的:探索胃癌病人血清中潜在的N-糖肽生物分子标志物。方法:采用超高效液相色谱串联三重四级杆质谱法(UPLC-QqQ-MS/MS)对胃癌病人和胃炎病人血清中的N-糖肽进行定量分析,通过独立样本t检验与偏最小二乘法(partialleast squares,PLS)对数据进行分析。结果:对约60种N-糖肽进行统计分析,找到7个具有显著性差异的N-糖肽,分别为N4400-IgG1、N5400-IgG1、N4410-IgG1、N3500-IgA、N5400-IgA、N5402-IgA和N5501-J。以这7种N-糖肽对测试组进行PLS分析,结果表明胃炎病人和胃癌病人之间已出现一定的分离趋势。结论:本研究发现了胃癌潜在的N-糖肽生物分子标志物。该方法为探索胃癌生物分子标志物提供了有力的技术支持,对胃癌的早期诊断具有重要临床意义。
Objective : To explore potential N-glycopeptide biomolecular markers in serum of gastric cancer patients . Methods :The N-glycopeptides in the serum samples of patients with gastric cancer and gastritis were quantitatively analyzed by ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QqQ-MS/MS).The data were analyzed by independent samples t-test and partial least squares (PLS) methods .Results : Seven N-glycopeptides with significant differences were found among about sixty different N-glycopeptides ,namely N4400-IgG1 ,N5400-IgG1 ,N4410-IgG1 , N3500-IgA ,N5400-IgA ,N5402-IgA and N5501-J .The results showed that there was a certain separation tendency between patients with gastritis and gastric cancer .Conclusion :Potential N-glycopeptides biomolecular markers for gastric cancer were identified .This method provides powerful technical support for exploring biomarkers of gastric cancer ,and is of important clini- cal significance for early diagnosis of gastric cancer .
作者
杨楠
范国荣
YANG Nan;FAN Guorong(Teaching and Research Section of Pharmaceutical Analysis ,School of Pharmcy ,Navy Military Medical University ,Shanghai Key Laboratory for Pharmaceutical (Chinese Materia Medica) Metabolite Research , Shanghai 200433 ,China;Department of Pharmacy ,Qingdao Special Service Sanatorium of Chinese Navy ,Shandong Qingdao266072 ,China;Laboratory of Drug Metabolism and Pharmacokinetics ,School of Medicine ,Tongji University ,Shanghai 200092 ,China;Department of Clinical Pharmacy ,Shanghai General Hospital ,Shanghai Jiaotong University ,Shanghai 200080 , China)
出处
《药学服务与研究》
CAS
2019年第1期19-24,共6页
Pharmaceutical Care and Research
关键词
胃癌
N-糖肽
生物分子标志物
色谱法
高效液相
质谱法
联用
gastric cancer
N-glycopeptide
biomolecular marker
chromatography ,high performance liquid
mass spectrometry ,tandem
