过表达PRDM13抑制胶质瘤细胞相关功能基因的测序分析

Sequencing Analysis of Inhibition Glioma Cell Related Functional Genes by Overexpression PRDM13

目的比较过表达PRDM13慢病毒与感染阴性对照病毒的胶质瘤细胞mRNA存在的差异,筛选并分析抑制胶质瘤增殖、迁移相关的基因。方法通过胶质瘤细胞感染慢病毒,建立过表达PRDM13细胞模型,使用转录组测序方法检测两组中差异表达基因,并进行GO分析。结果过表达PRDM13慢病毒细胞模型成功建立,过表达PRDM13可以改变U87细胞的形态,转录组测序检测到实验组和对照组显著性差异表达基因。结论找到抑制胶质瘤增殖、迁移的相关基因,为进一步研究PRDM13的作用机制提供了理论依据。

Objective To compare the expression differences of mRNA between PRDM13 and negative lentivirus infection virus in glioma cells, screening and analyzing the related genes of inhibition on proliferation and migration. Methods The glioma cells were infected with lentivirus, to establish cell model of overexpression PRDM13, using transcriptome sequencing to detect the difference between the two groups in the expression of genes. Then we made GO analysis. Results Overexpression PRDM13 lentivirus cell model was successfully established and it could change the morphology of U87 cells. Significant different expressed genes were detected by transcriptome sequencing in the experimental group and the control group. Conclusions This study finds the related genes of inhibition of glioma proliferation and migration. It provides a theoretical basis for the further study of the mechanism of the PRDM13.