载万古霉素MSNs复合硫酸钙制备人工骨的缓释与抗菌作用研究

Controlled release and antibacterial activity of vancomycin in artificial bone based on mesoporous silica nanoparticles and calcium sulfate composites

目的检测载万古霉素介孔二氧化硅纳米颗粒(MSNs)复合硫酸钙人工骨,延长万古霉素释放时间及其抑菌作用。方法分别制备相同载万古霉素量的Van-MSNs-CaSO4和Van-CaSO4颗粒,按0.1 g/mL比例浸泡于PBS溶液中,37℃恒温浸提,按不同时间点取浸提液,全量和半量更换新鲜PBS溶液,分别检测两种颗粒浸提液中的万古霉素含量,并取浸提液行抑菌试验,测量抑菌环大小,比较两种载药颗粒的持续释药时间及相同时间点浸提液的抑菌能力。结果全量更换PBS组Van-MSNs-CaSO4的释药时间为4 d,Van-CaSO4的释药时间为2 d;半量更换PBS组释药时间达35 d,Van-CaSO4的释药时间为14 d。抑菌环直径检测释出药物有抑菌作用。结论 Van-MSNs-CaSO4复合材料较Van-CaSO4可以明显延长万古霉素释放时间并具有抑菌活性。

Objective Investigate the sustained-release of vancomycin in artificial bone based on MSN-calcium sulfate composites and its bacteriostatic effect.Methods The Van-MSNs-CaSO4 and Van-CaSO4 particles with the same vancomycin content were prepared and soaked in PBS solution at 0.1 g/mL.Then it was extracted at constant temperature(37℃),and the extraction was collected at different time.The fresh PBS solution was replaced by a fulland half quantity,and the content of vancomycin in the two types of leaching solution wasmeasured.Then the extracts were used for bacteriostasis.The size of the bacteriostaticring was measured.The sustained-release time and the bacteriostatic ability of the extractsof two drug loaded granules were compared.Results First,the releasing time of Van-MSNs-CaSO4 in the PBS group was 4 days,while the Van-CaSO4 was 2 days.Second,the release time of Van-MSNs-CaSO4 by half amount replacement of PBS group was 35 days,and the Van-CaSO4 was 14 days.Third,the bacteriostatic ring showed that the released vancomycin had bacteriostatic effect.Conclusion Van-MSNs-CaSO4 composite can significantly prolong the releasing time of vancomycin than Van-CaSO4,without affecting antibacterial activity.