短期变温驯化对赤拟谷盗热适应性的影响

The Effect of Short-term Variable Thermal Acclimation on the Heat Adaptation of Tribolium Castaneum

先将赤拟谷盗各虫态在不同升温速率(0、10、5、2.5℃/h)下从28℃升至50℃经历短期变温驯化,然后观察记录其在50℃下不同处理时间(0、15、30、45、60 min)后的死亡率。研究结果表明:不同的处理时间、不同的升温速率对赤拟谷盗各虫态死亡率具有显著影响。在50℃下处理0～30 min时,经历变温驯化的赤拟谷盗卵死亡率比未经驯化的明显降低;在50℃下处理15～30min时,经历2.5℃/h变温驯化的赤拟谷盗幼虫死亡率显著低于经历其他变温速率驯化和对照组幼虫的死亡率;当处理时间为30 min时,赤拟谷盗蛹的死亡率随着变温驯化速率的减慢而逐渐上升,在50℃下处理15 min时,经历2.5℃/h变温速率驯化的蛹的死亡率为93.33%,显著高于其他变温速率驯化下蛹的死亡率;赤拟谷盗成虫的死亡率随着变温驯化速率的减缓而上升。

The eggs,larvae,pupae,and adults of Tribolium castaneum firstly experienced variable thermal acclimation at different heating rates (0,10,5 and 2.5 ℃/h) from 28 ℃ to 50 ℃,then they were treated at 50 ℃ for different times(0,15,30,45 and 60 min),and their mortality were investigated. The results showed that different treatment times and different heating rates significantly affected the mortality. When treated at 50 ℃ for 0-30 min,the mortality of T. castaneum eggs with acclimation was significantly lower than that of T. castaneum eggs without acclimation. When treated at 50 ℃ for 15-30 min,the mortality of T. castaneum larvae with variable thermal acclimation at 2.5 ℃/h was lowest. When the treatmet time was 30 min,the mortality of T. castaneum pupae increased with the decreasing heat rate. When treated at 50 ℃ for 15 min,the mortality of pupae acclimated at 2.5 ℃/h was 93.33%,which was significantly higher than other groups. The mortality rate of Tribolium castaneum adults increased with the decreasing heating rates.