七氟烷调节CARMA3靶向NF-κB通路抑制胃癌细胞迁移、侵袭

Sevoflurane regulates CARMA3 to inhibit migration and invasion of gastric cancer cells by targeting NF-κB signaling pathway

目的研究七氟烷对胃癌(gastric cancer, GC)细胞迁移、侵袭的影响,并探讨其作用机制.方法运用Transwell法检测细胞迁移、侵袭量;将siCAR MA3组(转染siCARMA3)、NC组(转染siControl)、CARMA3组(转染pcDNA3.1-CARMA3)、Vector组(转染pcDNA3.1)均用脂质体法转染至SGC7901细胞;用qRT-PCR检测细胞中CARMA3 mRNA表达;Westernblot检测细胞中CARMA3、p-p65、p65蛋白表达.结果与对照组相比,七氟烷抑制GC细胞迁移、侵袭,且抑制CARMA3表达;沉默CARMA3抑制GC细胞迁移、侵袭,过表达CARMA3促进GC细胞迁移、侵袭,且CARMA3靶向NF-κB通路;七氟烷可抑制CARMA3失活NF-κB通路抑制GC细胞迁移、侵袭.结论七氟烷可抑制GC细胞迁移、侵袭,其机制可能与失活CARMA3/NF-κB信号通路有关,将可为七氟烷用于临床治疗GC提供依据.

AIM To investigate the effect of sevoflurane on cell migration and invasion in gastric cancer(GC)cells,and to explore the underlying mechanism.METHODS After SGC7901 cells were transfected with siCARMA3(siCARMA3 group),siControl(NC group),pcDNA 3.1-CARMA3(CARMA3 group),or pcDNA 3.1(vector group)by liposome method,the expression of CARMA3 mRNA in cells was detected by qRT-PCR,and the protein expression of CARMA3,p-p65,and p65 was detected by Western blot.RESULTS Compared with the control group,sevoflurane inhibited the migration and invasion of GC cells and downregulated the expression of CARMA3.Silencing of CARMA3 inhibited the migration and invasion of GC cells,while overexpression of CARMA3 promoted the migration and invasion of GC cells.CARMA3 targeted the NF-κB pathway.Thus,sevoflurane regulated CARMA3 to inhibit migration and invasion of GC cells by targeting the NF-κB pathway.CONCLUSION Sevoflurane could inhibit the migration and invasion of GC cells via mechanisms that may be related to the regulation of CARMA3 to target the NF-κB pathway.This finding will provide a basis for clinical treatment of GC with sevoflurane.