忍冬藤饮片标准煎液质量评价研究

Study on Quality Evaluation of Standard Decoction of Lonicerae Japonicae Caulis Decoction Pieces

目的制备忍冬藤饮片标准煎液,并建立其质量控制方法。方法按照标准煎液制备要求制备15批忍冬藤饮片标准煎液,以绿原酸、咖啡酸、马钱苷作为定量检测指标,计算出膏率、含量和转移率,并建立超高液相色谱特征图谱分析方法。结果 15批忍冬藤饮片标准煎液的出膏率范围为6.44%～11.95%,绿原酸、咖啡酸、马钱苷含量范围分别为12.17～22.61 mg/g、2.75～5.12 mg/g、21.71～40.32 mg/g,转移率范围分别为28.13%～52.25%、53.93%～100.15%、40.04%～74.36%。采用《中药色谱指纹图谱相似度评价系统(2012版)》软件进行分析和相似度评价,标定了10个共有特征峰,并指认绿原酸、咖啡酸、马钱苷3个色谱峰。通过与对照图谱比对,相似度均高于0.96。结论本研究建立的忍冬藤饮片标准煎液的质量评价方法准确、稳定、重复性良好,可为忍冬藤配方颗粒的质量控制提供参考。

Objective To prepare standard decoction of Lonicerae Japonicae Caulis decoction pieces;To establish quality control method. Methods Fifteen batches of standard decoction of Lonicerae Japonicae Caulis decoction pieces were prepared according to the standardization method. With chlorogenic acid, caffeic acid and loganin as the detection indices, the extraction rate, content, and transfer rate were calculated and the UPLC characteristic chromatograms analysis method was established. Results According to the measurement of 15 batches of standard decoction of Lonicerae Japonicae Caulis decoction pieces, the extractum rate was at the range of 6.44%-11.95%. The contents of chlorogenic acid, caffeic acid and loganin were 12.17-22.61 mg/g, 2.75-5.12 mg/g and 21.71-40.32 mg/g, respectively. The transfer rates were 28.13%-52.25%, 53.93%-100.15% and 40.04%-74.36%, respectively. Besides, Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012 edition) was used to analyze and compare the characteristic chromatograms. Ten common peaks were determined, and three chromatographic peaks of chlorogenic acid, caffeic acid, and loganin were identified. The similarity was over 0.96 by comparing with the control map. Conclusion This study establishes the quality control method of standard decoction of Lonicerae Japonicae Caulis decoction pieces, which is accurate, stable, and with good repeatability, which can provide references for the quality control of Lonicerae Japonicae Caulis formula granules.