脑脊液和海马组织儿茶酚胺氧位甲基转移酶与阿尔茨海默病模型大鼠学习记忆功能的相关性研究

The correlation between catechol O-methyl-transferase in cerebrospinal fluid and hippocampal and learning and memory function in rats with Alzheimer disease

目的探讨脑脊液和海马组织儿茶酚胺氧位甲基转移酶(catechol O-methyl-transferase,COMT)与阿尔茨海默病(Alzheimer disease,AD)模型大鼠学习记忆功能的相关性。方法选取40只健康雄性SD老年大鼠,随机分为假手术组(Sham)和阿尔茨海默病模型组(Model),每组20只,检测动物回避电击逃避行为潜伏期及错误反映次数,观察阿尔茨海默病模型大鼠学习记忆功能等行为学的变化,测定各组大鼠脑脊液和海马组织中COMT的mRNA和蛋白表达情况。结果 (1)相较于假手术组,阿尔茨海默病模型组大鼠的潜伏期显著缩短,错误次数显著增加(P <0. 01);(2)相较于假手术组,阿尔茨海默病模型组大鼠脑脊液和海马组织中COMT的mRNA表达量均显著升高,蛋白表达量显著上调(P <0. 01)。结论阿尔茨海默病可致大鼠的学习记忆功能严重损伤,并上调其脑脊液和海马组织中COMT的mRNA和蛋白表达量,证实脑脊液和海马组织儿茶酚胺氧位甲基转移酶与阿尔茨海默病模型大鼠学习记忆功能具有一定相关性。

Objective To investigate the relationship between COMT in CSF and hippocampal tissue and the learning and memory function of AD rats.Methods Forty healthy male SD aged rats were randomly divided into sham group and model group with 20 rats in each group.The latent period of avoidance of electric shock and the number of erroneous reactions were detected.The changes of learning and memory function were observed.The expression of COMT in cerebrospinal fluid and hippocampal tissue of rats in each group were determined.Results (1)Compared with sham-operated group,the latency of rats in Alzheimer’s disease model group was significantly shortened and the number of errors significantly increased (P<0.01);(2)Compared with sham-operated group,the expression of COMT in cerebrospinal fluid and hippocampus of rats in Alzheimer’s disease model group was significantly increased (P<0.01).Conclusion Alzheimer’s disease can cause severe impairment of learning and memory function in rats,and up-regulate the expression of COMT in CSF and hippocampus,which confirms that catecholamine O methyltransferase in CSF and hippocampus is related to learning and memory function in rats with Alzheimer’s disease.