侵染广东顺德2种兰花的辣椒褪绿病毒分子鉴定

Molecular Identification of Capiscum chlorosis virus Infected Two Species of Orchids in Shunde, Guangdong Province

[目的]对侵染蝴蝶兰和文心兰的辣椒褪绿病毒进行田间调查,并进行分子鉴定,为广东顺德花卉顺利出口提供技术支持和把关服务。[方法]根据辣椒褪绿病毒的一对特异性引物核壳体(nucleocapsid,N)基因,应用RT-PCR检测方法对2种兰花疑似感染病毒的植株进行分子鉴定。[结果]蝴蝶兰(样品编号2、3、5和10)和文心兰(样品编号6)感染辣椒褪绿病毒,系统进化树表明2种兰花感染的辣椒褪绿病毒与该病毒泰国分离物以较高的后验概率聚为同一类群。[结论]辣椒褪绿病毒已在广东顺德的蝴蝶兰和文心兰上发生危害,且文心兰感染辣椒褪绿病毒为国内首次报道,出口企业需加强检疫监控工作。

[Objective]Field investigation and molecular identification of CaCV infected with Phalaenopsis and Oncidium were conducted,to provide technical supports and security services for the smooth export of flowers in Shunde,Guangdong.[Method]Based on the nucleocapsid (N) gene of a pair of specific primers of CaCV,the two species of orchids with suspected virus infection were identified by RT-PCR.[Result] Phalaenopsis (sample No.2,3,5 and 10) and Oncidium (sample No.6) were infected with CaCV.Phylogenetic tree showed that the two orchids infected with CaCV and the Thai isolate of the virus were clustered into the same group with higher posterior probability.[Conclusion] Capiscum chlorosis virus has been harmful in Phalaenopsis and Oncidium in Shunde,Guangdong Province,the infection of Oncidium with CaCV is the first reported in China.Export enterprises need to strengthen quarantining and monitoring work.