摘要
脱落酸(ABA)是一种内源性激素,在调节植物的生长发育、非生物胁迫和生物胁迫反应方面发挥着重要作用。Pyrabactin resistance 1-like(PYR/PYL)蛋白在ABA信号传导途径中起核心调控作用。然而,在蒺藜苜蓿(Medicagotruncatula)中并没有关于这个家族的细节。本研究根据拟南芥(Arabidopsis thaliana)中的14个PYL基因,利用BLAST方法鉴定蒺藜苜蓿基因组中的PYL家族成员。结果表明,鉴定得到14个蒺藜苜蓿PYL基因家族成员,且所有成员均含有PYR-PYL-RCAR-like功能域。预测启动子中的顺式元件发现所有蒺藜苜蓿PYL基因家族成员均含有与胁迫以及激素相关的元件。基因本体论(GO)富集分析与KEGG通路分析显示其主要参与到脱落酸信号通路及对外界刺激的反应。基因表达模式分析显示,其在逆境胁迫下表现出特定的表达模式。本研究为进一步研究蒺藜苜蓿PYL基因奠定了基础。
Abscisic acid (ABA) is an endogenous hormone that plays an important role in regulating plant growth and development, and abiotic stress and biotic stress responses. Pyrabactin resistance 1-like (PYR/PYL) protein is a core regulatory component of the ABA signaling pathway. However, details about this family in Medicago truncatula are unknown. This study used BLAST method to identify members of the PYL family in the M. truncatula genome based on the 14 PYL genes in Arabidopsis thaliana. Consequently, 14 members of the PYL gene family were identified, all of which contain the PYR-PYL-RCAR-like domain. The cis-elements in the predicted promoters revealed that all the members of PYL gene family contain elements associated with stress and hormones. Gene Ontology (GO) enrichment analysis and KEGG pathway analysis show that members of the PYL family are mainly involved in the abscisic acid signaling pathway and that they respond to external stimuli. Analysis of gene expression patterns showed that they exhibit specific expression patterns under stress. This study lays the foundation for further studies on the PYL genes in M. truncatula.
作者
黄思源
呼天明
杨培志
HUANG Siyuan;HU Tianming;YANG Peizhi(College of Grass and Grassland,Northwest A & F University,Yangling 712100,Shaanxi,China)
出处
《草业科学》
CAS
CSCD
2019年第2期422-433,共12页
Pratacultural Science
基金
国家自然科学基金(31572456)
国家现代农业产业技术体系(CARS-34)
关键词
蒺藜苜蓿
脱落酸
PYL
基因表达模式
非生物胁迫
Medicago truncatula
abscisic acid
PYL
gene expression pattern
abiotic stress