非降解聚氨酯弹性体支架材料体外构建残耳软骨用于修复耳廓组织的可行性

Feasibility of Using Non-degradable Polyurethane Elastomer Scaffold to Construct Residual Ear Cartilage for Repairing Auricular Tissue in Vitro

目的:探讨以非降解聚氨酯弹性体支架材料体外构建残耳软骨用于修复耳廓组织的可行性。方法:分离30例临床小儿畸形患者软骨细胞,经培养、扩增后制成细胞悬液滴加于圆盘状聚氨酯支架上,体外培养21d后接种于裸鼠体内培养42d,应用扫描电镜观察细胞-支架复合物生长情况,同时使用免疫组织化学法观察成软骨细胞冰冻切片情况。结果:软骨细胞体外培养3周后形成的细胞-支架复合物的形态学及组织学较正常耳廓软骨组织相差甚远,而在裸鼠体内培养6周的细胞-支架复合物外观光滑细腻,与正常耳廓软骨组织相近,经免疫组织化学法分析可见新生的软骨组织细胞间质中存在蓝黑色的丝网状弹力纤维。结论:聚氨酯材料能够成为组织工程耳廓软骨的支架材料,且体内培养较体外培养更利于软骨组织的生长。

Objective To investigate the feasibility of constructing tissue engineered cartilage in vitro by using chondrocytes and non-degradable polyurethane elastomers as clinical scaffolds in vitro. Methods 30 cases of rabbit chondrocytes were isolated from clinical pediatric malformation. The cell suspension was added to disc-shaped polyurethane scaffold after culture and amplification after vitro culture for 21 days. They were inoculated into nude mice for 42 days. The growth of the cell scaffold complex was observed by electron microscopy. The frozen sections of osteoblasts were observed by immunohistochemistry. Results The morphology and histology of the cell-scaffold complex formed by chondrocytes cultured for 3 weeks in vitro was much different from that of normal auricular cartilage tissue, while the cell-scaffold complex cultured in nude mice for 6 weeks was smooth and delicate. Similar to the normal auricular cartilage tissue, immunohistochemical analysis showed that there were blue-black mesh-like elastic fibers in the interstitial cells of the new cartilage tissue. Conclusion Polyurethane material can be used as a scaffold material for tissue engineering auricular cartilage, and in vivo culture is more conducive to the growth of cartilage tissue than in vitro culture.