超声联合酶法提取玫瑰花总黄酮工艺条件优化

Optimization on Extraction Technology of Flavonoids from Rosa rugosa by Ultrasound Combined with Cellulose Enzyme

目的:探索超声联合酶法提取玫瑰花总黄酮的最佳工艺条件。方法:以总黄酮提取率作为评价指标,分别考察酶解温度、酶质量分数、固液比、乙醇浓度、超声提取时间及超声提取温度对提取效果的影响。在单因素考察的基础上,采用正交试验设计优化提取工艺条件。结果:优化得到的工艺参数为固液比1∶25,酶解温度50℃,酶质量分数0. 4%,超声时间30 min。结论:优选的工艺合理、可行,具有提取率高、节能等优点,可为工业化生产提供参考。

Objective:The extraction process of flavonoids in Rosa rugosa by ultrasound combined with cellulose enzyme was optimized.Methods:The extraction rate of flavonoids was used as index.Based on the effects of some factors such as enzymatic temperature,enzyme mass concentration,ratio to solid,the concentration of ethanol,ultrasonic time and ultrasonic temperature,orthogonal design was applied to decide the best extraction process.Results:The optimal extraction technology was as follows:ratio to solid was 1 :25,enzymatic temperature was 50 ℃,enzyme mass concentration was 0.4%,ultrasonic time was 30 min.Conclusion:The optimized process is reasonable and feasible,which can enhance extract-efficiency and is energy-saving.It provides reference for industrial production.